QQ客服 官方微博 反馈留言

	Identification and quantitative analysis of significantly accumulated proteins during the Arabidopsis seedling de-etiolation process
	Wang, BC ; Pan, YH ; Meng, DZ ; Zhu, YX
	2006
关键词	Arabidopsis de-etiolation light two-dimensional electrophoresis LIGHT CONTROL THALIANA EXPRESSION GENES
英文摘要	Proteomic analysis was performed on seedlings after different light treatments. A total of (1 350 31) protein spots was separated and visualized on each silver nitrate-stained two-dimensional gel using protein samples prepared from light-grown or etiolated seedlings with or without 6-9 h light treatment. Twenty-five protein spots (encoded by 19 genes) that were significantly accumulated upon light treatment were identified using the matrix-assisted laser desorption ionization time-of-flight mass spectrometry method. Functional proteomics indicated that these proteins involved mainly in chloroplast development, energy metabolism, cell cycle progression and membrane electron transport. For 18 of the protein-coding genes we identified through an internet search, the transcript levels of 17 genes matched roughly with their protein content in etiolated and green seedlings, suggesting that these genes were regulated by light mainly at the transcriptional level. Despite a very significant increase in the amount of proteins upon light treatment, similar RNA levels were found in dark-grown or green seedlings for the carbonic anhydrase gene At3g05100, indicating a possible post-transcriptional regulatory mechanism. Elucidation of light-induced protein accumulation will undoubtedly enhance our understanding of plant photomorphogenesis.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000234543100012&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Plant Sciences; SCI(E); CPCI-S(ISTP); 11
语种	英语
DOI标识	10.1111/j.1744-7909.2006.00215.x
内容类型	其他
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/252930]
专题	生命科学学院
推荐引用方式 GB/T 7714	Wang, BC,Pan, YH,Meng, DZ,et al. Identification and quantitative analysis of significantly accumulated proteins during the Arabidopsis seedling de-etiolation process. 2006-01-01.

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。