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	Phage-displayed protein chip based on SPR sensing
	Liu, Fangfang ; Luo, Zhaofeng ; Ding, Xiang ; Zhu, Shenggeng ; Yu, Xinglong
	2009
关键词	Surface plasmon resonance (SPR) Phage display Phage-displayed protein chip Kinetic parameters ANTIBODY PROTEOMICS
英文摘要	Surface plasmon resonance and phage display technique were combined to acquire abundant specific capture molecules and realize label-free and high-sensitive detection for the protein chip. The 12-amino acid peptide displayed on phage M13 coated protein pIII (ET101-4-11) was selected as the probe, it was immobilized on 11-mercapto-undecanoic acid (11-MUA) sensor chip to fabricate the phage-displayed protein chip, and the interaction between the peptide and the specific protein (ET101) was detected on the BIAcore3000. Experimental results show that when ET101 diluted in series concentrations flow through the sensor chip, the relationship between the response and the sample concentration fits the sigmoid-curve very well, and the ET101 concentration of 4.0 mu M corresponds to the response of 365RU +/- 8RU. The kinetic parameters are also calculated, including the association rate 4.75 M(-1) s(-1) and the dissociation rate 2.76 x 10(-3) s(-1). The research suggests that the phage-displayed protein chip can act as a useful tool in proteomics research. (C) 2008 Elsevier B.V. All rights reserved.; Chemistry, Analytical; Electrochemistry; Instruments & Instrumentation; SCI(E); EI; CPCI-S(ISTP); 7
语种	英语
DOI标识	10.1016/j.snb.2008.11.031
内容类型	其他
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/246530]
专题	生命科学学院
推荐引用方式 GB/T 7714	Liu, Fangfang,Luo, Zhaofeng,Ding, Xiang,et al. Phage-displayed protein chip based on SPR sensing. 2009-01-01.

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