Wnt proteins regulate acetylcholine receptor clustering in muscle cells

doi:10.1186/1756-6606-5-7

CORC > 北京大学 > 生命科学学院

	Wnt proteins regulate acetylcholine receptor clustering in muscle cells
	Zhang, Bin ; Liang, Chuan ; Bates, Ryan ; Yin, Yiming ; Xiong, Wen-Cheng ; Mei, Lin
刊名	molecular brain
	2012
关键词	Wnt AChR clustering muscle cells synapse formation neuromuscular junction NEUROMUSCULAR-JUNCTION POSTSYNAPTIC DIFFERENTIATION IN-VIVO AGRIN MUSK EXPRESSION SYNAPSE WINGLESS RAPSYN LRP4
DOI	10.1186/1756-6606-5-7
英文摘要	Background: The neuromuscular junction (NMJ) is a cholinergic synapse that rapidly conveys signals from motoneurons to muscle cells and exhibits a high degree of subcellular specialization characteristic of chemical synapses. NMJ formation requires agrin and its coreceptors LRP4 and MuSK. Increasing evidence indicates that Wnt signaling regulates NMJ formation in Drosophila, C. elegans and zebrafish. Results: In the study we systematically studied the effect of all 19 different Wnts in mammals on acetylcholine receptor (AChR) cluster formation. We identified five Wnts (Wnt9a, Wnt9b, Wnt10b, Wnt11, and Wnt16) that are able to stimulate AChR clustering, of which Wnt9a and Wnt11 are expressed abundantly in developing muscles. Using Wnt9a and Wnt11 as example, we demonstrated that Wnt induction of AChR clusters was dose-dependent and non-additive to that of agrin, suggesting that Wnts may act via similar pathways to induce AChR clusters. We provide evidence that Wnt9a and Wnt11 bind directly to the extracellular domain of MuSK, to induce MuSK dimerization and subsequent tyrosine phosphorylation of the kinase. In addition, Wnt-induced AChR clustering requires LRP4. Conclusions: These results identify Wnts as new players in AChR cluster formation, which act in a manner that requires both MuSK and LRP4, revealing a novel function of LRP4.; Neurosciences; SCI(E); 24; ARTICLE; 5
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/234692]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhang, Bin,Liang, Chuan,Bates, Ryan,et al. Wnt proteins regulate acetylcholine receptor clustering in muscle cells[J]. molecular brain,2012.
APA	Zhang, Bin,Liang, Chuan,Bates, Ryan,Yin, Yiming,Xiong, Wen-Cheng,&Mei, Lin.(2012).Wnt proteins regulate acetylcholine receptor clustering in muscle cells.molecular brain.
MLA	Zhang, Bin,et al."Wnt proteins regulate acetylcholine receptor clustering in muscle cells".molecular brain (2012).