Transcriptome profiling confirmed correlations between symptoms and   transcriptional changes in RDV infected rice and revealed nucleolus as a   possible target of RDV manipulation

doi:10.1186/1743-422X-11-81

CORC > 北京大学 > 生命科学学院

	Transcriptome profiling confirmed correlations between symptoms and transcriptional changes in RDV infected rice and revealed nucleolus as a possible target of RDV manipulation
	Yang, Liang ; Du, Zhenguo ; Gao, Feng ; Wu, Kangcheng ; Xie, Lianhui ; Li, Yi ; Wu, Zujian ; Wu, Jianguo
刊名	virology journal
	2014
关键词	RDV Transcriptome profiling Pns11 Nucleolus MAJOR STRUCTURAL PROTEINS OUTER CAPSID PROTEIN VIRUS-LIKE PARTICLES DWARF-VIRUS ARABIDOPSIS-THALIANA GENE-EXPRESSION INSECT VECTOR RNA VIRUSES PLANTS GENOME
DOI	10.1186/1743-422X-11-81
英文摘要	Background: Rice dwarf virus (RDV) is the causal agent of rice dwarf disease, which limits rice production in many areas of south East Asia. Transcriptional changes of rice in response to RDV infection have been characterized by Shimizu et al. and Satoh et al.. Both studies found induction of defense related genes and correlations between transcriptional changes and symptom development in RDV-infected rice. However, the same rice cultivar, namely Nipponbare belonging to the Japonic subspecies of rice was used in both studies. Methods: Gene expression changes of the indica subspecies of rice, namely Oryza sativa L. ssp. indica cv Yixiang2292 that show moderate resistance to RDV, in response to RDV infection were characterized using an Affymetrix Rice Genome Array. Differentially expressed genes (DEGs) were classified according to their Gene Ontology (GO) annotation. The effects of transient expression of Pns11 in Nicotiana benthaminana on the expression of nucleolar genes were studied using real-time PCR (RT-PCR). Results: 856 genes involved in defense or other physiological processes were identified to be DEGs, most of which showed up-regulation. Ribosome-and nucleolus related genes were significantly enriched in the DEGs. Representative genes related to nucleolar function exhibited altered expression in N. benthaminana plants transiently expressing Pns11 of RDV. Conclusions: Induction of defense related genes is common for rice infected with RDV. There is a co-relation between symptom severity and transcriptional alteration in RDV infected rice. Besides ribosome, RDV may also target nucleolus to manipulate the translation machinery of rice. Given the tight links between nucleolus and ribosome, it is intriguing to speculate that RDV may enhance expression of ribosomal genes by targeting nucleolus through Pns11.; Virology; SCI(E); 0; ARTICLE; wuzujian@126.com; wujianguo81@126.com; 11
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/212789]
专题	生命科学学院
推荐引用方式 GB/T 7714	Yang, Liang,Du, Zhenguo,Gao, Feng,et al. Transcriptome profiling confirmed correlations between symptoms and transcriptional changes in RDV infected rice and revealed nucleolus as a possible target of RDV manipulation[J]. virology journal,2014.
APA	Yang, Liang.,Du, Zhenguo.,Gao, Feng.,Wu, Kangcheng.,Xie, Lianhui.,...&Wu, Jianguo.(2014).Transcriptome profiling confirmed correlations between symptoms and transcriptional changes in RDV infected rice and revealed nucleolus as a possible target of RDV manipulation.virology journal.
MLA	Yang, Liang,et al."Transcriptome profiling confirmed correlations between symptoms and transcriptional changes in RDV infected rice and revealed nucleolus as a possible target of RDV manipulation".virology journal (2014).