Construction and characterization of trifunctional single-chain   urokinase-type plasminogen activators - Trifunctional plasminogen   activators

doi:10.1046/j.1432-1327.2002.02816.x

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	Construction and characterization of trifunctional single-chain urokinase-type plasminogen activators - Trifunctional plasminogen activators
	Bi, Q ; Cen, XD ; Huang, YX ; Zhu, SG
刊名	european journal of biochemistry
	2002
关键词	scu-PA hirudin RGD motif fibrinolytic activity antithrombin activity THROMBIN
DOI	10.1046/j.1432-1327.2002.02816.x
英文摘要	Two chimeric proteins have been constructed. One consists of four parts: a portion of the low molecular mass single-chain urokinase-type plasminogen activator (scu-PA-32K, residues 144-411), a 15-mer linker sequence, the C-terminal amino-acid sequence (residues 53-65) of hirudin (Hir), and an RGD sequence derived from the leech protein decorsin, i.e. scu-PA(32 k)-linker-Hir (residues 53-65)-RGD peptide. The other comprises two main segments: scu-PA(32 k) and hirudin into which RGDSP is inserted between its residues 33 and 34, i.e. hirudin (residues 1-33)-RGDSP-hirudin (residues 34-65)-scu-PA(32 k). These two chimeric genes were expressed in Escherichia coli, and the products were purified by Zn2+-chelating Sepharose 4B chromatography and benzamidine Sepharose 6B chromatography. Our results suggested that these two chimeric proteins not only had plasminogen-dependent fibrinolytic activity, but also possessed platelet aggregation inhibitory activity and antithrombin activity.; Biochemistry & Molecular Biology; SCI(E); 9; 6; 1708-1713; 269
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/209813]
专题	生命科学学院
推荐引用方式 GB/T 7714	Bi, Q,Cen, XD,Huang, YX,et al. Construction and characterization of trifunctional single-chain urokinase-type plasminogen activators - Trifunctional plasminogen activators[J]. european journal of biochemistry,2002.
APA	Bi, Q,Cen, XD,Huang, YX,&Zhu, SG.(2002).Construction and characterization of trifunctional single-chain urokinase-type plasminogen activators - Trifunctional plasminogen activators.european journal of biochemistry.
MLA	Bi, Q,et al."Construction and characterization of trifunctional single-chain urokinase-type plasminogen activators - Trifunctional plasminogen activators".european journal of biochemistry (2002).