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	Characterization of HetR protein turnover in Anabaena sp. PCC 7120
	Zhou, RB ; Cao, ZX ; Zhao, JD
	1998
关键词	Anabaena cyanobacteria heterocyst HetR protein immunoblot overexpression pattern formation BLUE-GREEN-ALGA HETEROCYST DIFFERENTIATION PATTERN FORMATION CYANOBACTERIA CULTURES
DOI	10.1007/s002030050592
英文摘要	The hetR gene plays an important role in heterocyst development and pattern formation in heterocystous cyanobacteria. The hetR gene from Anabaena sp. PCC 7120 was overexpressed in Escherichia coli. Antibodies raised against the recombinant HetR protein (rHetR) were used to characterize metabolism of the HetR of Anabaena sp. PCC 7120 in vivo. HetR was present at a low level when Anabaena sp. PCC 7120 was grown in the presence of combined nitrogen. Shifting from nitrogen repletion conditions to nitrogen depletion conditions led to a two fold increase of HetR in total cell extracts, and most of HetR was located in heterocysts. The amount of HetR in total cellular extracts increased rapidly after shifting to nitrogen depletion conditions and reached a maximum level 3 h after the shift. Isoelectrofocusing electrophoresis revealed that the native HetR had a more acidic isoelectric point than did rHetR. After combined nitrogen was added to the nitrogen-depleted cultures, the degradation of HetR depended on culture conditions: before heterocysts were fully developed, HetR was rapidly degraded; after heterocysts were fully developed, HetR was degraded much more slowly. The distribution of HetR in other species of cyanobacteria was also studied.; Microbiology; SCI(E); PubMed; 35; ARTICLE; 5; 417-423; 169
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/202027]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zhou, RB,Cao, ZX,Zhao, JD. Characterization of HetR protein turnover in Anabaena sp. PCC 7120[J],1998.
APA	Zhou, RB,Cao, ZX,&Zhao, JD.(1998).Characterization of HetR protein turnover in Anabaena sp. PCC 7120..
MLA	Zhou, RB,et al."Characterization of HetR protein turnover in Anabaena sp. PCC 7120".(1998).

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