Construction, expression, and characterization of recombinant hirudin in   Escherichia coli

doi:10.1385/ABAB:95:1:23

CORC > 北京大学 > 生命科学学院

	Construction, expression, and characterization of recombinant hirudin in Escherichia coli
	Bi, Q ; Zhang, JS ; Huang, YX ; Su, HJ ; Zhou, XW ; Zhu, SG
刊名	应用生物化学与生物技术
	2001
关键词	antithrombin expression purification recombinant hirudin HV2-K47 secretion SECRETION CLONING
DOI	10.1385/ABAB:95:1:23
英文摘要	The mutant gene of HV2-K47 was obtained by polymerase chain reaction-directed mutagenesis and expressed in Escherichia coli. Many elements that could affect its expression level were compared. The product was purified to homogeneity via three chromatographic steps-ion exchange, gel filtration, and reverse phase chromatography-on the AKTA Explorer System. The antithrombin activity of HV2-K47 is much higher than that of recombinant HV2. Some properties and expression conditions were investigated systematically, which would be useful for further studies of hirudin and other small proteins.; Biochemistry & Molecular Biology; Biotechnology & Applied Microbiology; SCI(E); PubMed; 0; ARTICLE; 1; 23-30; 95
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/200530]
专题	生命科学学院
推荐引用方式 GB/T 7714	Bi, Q,Zhang, JS,Huang, YX,et al. Construction, expression, and characterization of recombinant hirudin in Escherichia coli[J]. 应用生物化学与生物技术,2001.
APA	Bi, Q,Zhang, JS,Huang, YX,Su, HJ,Zhou, XW,&Zhu, SG.(2001).Construction, expression, and characterization of recombinant hirudin in Escherichia coli.应用生物化学与生物技术.
MLA	Bi, Q,et al."Construction, expression, and characterization of recombinant hirudin in Escherichia coli".应用生物化学与生物技术 (2001).