The hydrophobic surface of PaAMP from pokeweed seeds is essential to its   interaction with fungal membrane lipids and the antifungal activity

doi:10.1016/j.febslet.2005.03.046

CORC > 北京大学 > 生命科学学院

	The hydrophobic surface of PaAMP from pokeweed seeds is essential to its interaction with fungal membrane lipids and the antifungal activity
	Peng, C ; Dong, CX ; Hou, QM ; Xu, CY ; Zhao, JD
刊名	欧洲生化学会联合会快报
	2005
关键词	antimicrobial protein pokeweeds site-specific mutagenesis sphingolipids DAHLIA DAHLIA-MERCKII MIRABILIS-JALAPA L ANTIMICROBIAL PEPTIDES PLANT DEFENSINS SACCHAROMYCES-CEREVISIAE CLONING COMPONENTS GENE
DOI	10.1016/j.febslet.2005.03.046
英文摘要	PaAMP is a small seed-specific antimicrobial protein from pokeweeds. It has a cysteine-knot fold with a positive patch and a hydrophobic surface. Site-specific mutagenesis was performed to study the roles of these two domains in antimicrobial activity and we found that the mutations in the hydrophobic surface had a more profound effect than that in the positive patch. A protein-membrane interaction was observed with the green fluorescence protein-PaAMP (GFP-AMP) fusion protein. The mutations that replace the amino acid residues forming hydrophobic surface with neutral residues abolished the interaction of PaAMP with the membrane and the binding of PaAMP to fungal sphingolipids while ergosterol enhanced the binding, suggesting that the hydrophobic surface was required for the interaction between PaAMP and fungal plasma membrane lipid raft. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.; Biochemistry & Molecular Biology; Biophysics; Cell Biology; SCI(E); PubMed; 0; ARTICLE; 11; 2445-2450; 579
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/199777]
专题	生命科学学院
推荐引用方式 GB/T 7714	Peng, C,Dong, CX,Hou, QM,et al. The hydrophobic surface of PaAMP from pokeweed seeds is essential to its interaction with fungal membrane lipids and the antifungal activity[J]. 欧洲生化学会联合会快报,2005.
APA	Peng, C,Dong, CX,Hou, QM,Xu, CY,&Zhao, JD.(2005).The hydrophobic surface of PaAMP from pokeweed seeds is essential to its interaction with fungal membrane lipids and the antifungal activity.欧洲生化学会联合会快报.
MLA	Peng, C,et al."The hydrophobic surface of PaAMP from pokeweed seeds is essential to its interaction with fungal membrane lipids and the antifungal activity".欧洲生化学会联合会快报 (2005).