金属硫蛋白-3对dUTPase调节dUTP细胞毒性作用的影响

doi:10.3321/j.issn:1000-3061.2004.03.015

CORC > 北京大学 > 生命科学学院

	金属硫蛋白-3对dUTPase调节dUTP细胞毒性作用的影响; Affection of Metallothionein-3 to dUTPase's Accommodating Cellular Toxicity of dUTP
	陈巧林 ; 康巧华 ; 任宏伟 ; 王宗元 ; 茹炳根
刊名	生物工程学报
	2004
关键词	hMT-3 dUTPase 基因转染 dUTP 细胞毒性
DOI	10.3321/j.issn:1000-3061.2004.03.015
英文摘要	金属硫蛋白-3(MT-3),又称神经生长抑制因子,是一种脑特异性金属硫蛋白.人细胞核dUTP焦磷酸酶(dUTP pyrophosphatase,dUTPase)是最近在脑中研究发现的能与人金属硫蛋白-3(human metallothionein-3,hMT-3)相互作用的一个蛋白,两者共同作用具有神经元生长抑制活性.为了探讨hMT-3对dUTPase调节dUTP引起的细胞毒性作用的影响,通过基因转染HEK293细胞观察细胞在dUTP中的生长情况,发现共转染hMT-3和dUTPase基因的细胞比单转染dUTPase或hMT-3基因的细胞具有更强的对dUTP细胞毒性的耐受能力;同时在大肠杆菌BL21中表达重组蛋白,测定hMT-3在dUTPase水解dUTP中的作用,结果显示重组蛋白hMT-3可以促进dUTPase对dUTP的水解.结果均初步证实了hMT-3对dUTPase抵抗dUTP引起的正常细胞死亡有一定的协同作用,为进一步研究dUTPase及其相互作用蛋白hMT-3在化疗中的应用提供理论依据.; Metallothionein-3 (MT-3), renamed as growth inhibitory factor (GIF), is a brain specific member of the metallothionein family. Human dUTPase is a recently found protein in brain that can interact with hMT-3. They have the growth inhibitory activity on neuron cell by interaction. To study the affection of hMT-3 to dUTPase's eliminating the cellular toxicity caused by dUTP, the pSVHA-dUTPase and pFLag-hMT-3 genes have been transfected into HEK293 cells. In addition, the dUTPase and hMT-3 proteins were expressed in BL21 to study the role of hMT-3 on the hydrolyzation of dUTP by dUTPase. The results demonstrate that the cells co-transfected with dUTPase and hMT-3 genes have more strong resistibility to dUTP than the cells transfected only with dUTPase gene. And that the hMT-3 protein can accelerate the hydrolyzation of dUTP by dUTPase. All these indicate that hMT-3 can cooperate with dUTPase to protect better the 293 cells from dUTP. This research offered the theoretic elements for the application of hMT-3 and dUTPase in chemic cure.; 国家科技攻关项目; PubMed; 中文核心期刊要目总览(PKU); 中国科学引文数据库(CSCD); 0; 3; 389-393; 20
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/199667]
专题	生命科学学院
推荐引用方式 GB/T 7714	陈巧林,康巧华,任宏伟,等. 金属硫蛋白-3对dUTPase调节dUTP细胞毒性作用的影响, Affection of Metallothionein-3 to dUTPase's Accommodating Cellular Toxicity of dUTP[J]. 生物工程学报,2004.
APA	陈巧林,康巧华,任宏伟,王宗元,&茹炳根.(2004).金属硫蛋白-3对dUTPase调节dUTP细胞毒性作用的影响.生物工程学报.
MLA	陈巧林,et al."金属硫蛋白-3对dUTPase调节dUTP细胞毒性作用的影响".生物工程学报 (2004).