Minichromosome maintenance protein 5 homologue in Caenorhabditis elegans   plays essential role for postembryonic development

doi:10.1016/j.bbrc.2007.05.203

CORC > 北京大学 > 生命科学学院

	Minichromosome maintenance protein 5 homologue in Caenorhabditis elegans plays essential role for postembryonic development
	Wang, Xiangming ; Suh, Christopher ; Zhu, Zuoyan ; Fan, Qichang
刊名	生物化学与生物物理学研究通讯
	2007
关键词	Caenorhabditis elegans MCM2-7 family mcm-5 cell division development C-ELEGANS DNA-REPLICATION CELL-DIVISION MCM PROTEINS CYCLE
DOI	10.1016/j.bbrc.2007.05.203
英文摘要	Genome duplication is tightly controlled in multicellular organisms to ensure the genome stability. Studies in Saccharomyces cerevisiae and Xenopus show that minichromosome maintenance (MCM) proteins are essential for genome duplication. However, the development role of MCM proteins in multicellular organisms is not well known. MCM5 encodes a member of the MCM2-7 protein family involved in the initiation of DNA replication. The sequences of all Mcm5 homologues from yeast to human are highly conserved and suggest that their functions are also conserved. Here, we isolated the first mutant allele of mcm-5 (fw7) in Caenorhabditis elegans. Homozygous mcm-5 (fw7) mutants from heterozygous parents exhibited variable larval lethality and adult sterility. The postembryonically born neuron number was decreased and also showed aberrant axon morphology. Our study revealed that the losses of neurons in mcm-5 (fw7) mutants were caused by cell cycle defects not by programmed cell death. The examination showed that mcm-5 was widely used for postembryonic development in multiple cells such as seam cells, gonad and intestinal cells. Knockdown of mcm-5 by RNAi caused 98.1% embryonic arrest, suggesting that mcm-5 was also required for embryonic development. After RNAi treatment of the other MCM2-7 family members, we found that they all exhibited similar phenotypes as mcm-5, suggesting that the MCM2-7 family in C elegans might function associated with cell division as its homologues in S. cerevisiae. (c) 2007 Elsevier Inc. All rights reserved.; Biochemistry & Molecular Biology; Biophysics; SCI(E); PubMed; 2; ARTICLE; 4; 965-971; 359
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/198309]
专题	生命科学学院
推荐引用方式 GB/T 7714	Wang, Xiangming,Suh, Christopher,Zhu, Zuoyan,et al. Minichromosome maintenance protein 5 homologue in Caenorhabditis elegans plays essential role for postembryonic development[J]. 生物化学与生物物理学研究通讯,2007.
APA	Wang, Xiangming,Suh, Christopher,Zhu, Zuoyan,&Fan, Qichang.(2007).Minichromosome maintenance protein 5 homologue in Caenorhabditis elegans plays essential role for postembryonic development.生物化学与生物物理学研究通讯.
MLA	Wang, Xiangming,et al."Minichromosome maintenance protein 5 homologue in Caenorhabditis elegans plays essential role for postembryonic development".生物化学与生物物理学研究通讯 (2007).