Crystal Structures of Cobalamin-Independent Methionine Synthase (MetE)   from Streptococcus mutans: A Dynamic Zinc-Inversion Model

doi:10.1016/j.jmb.2011.08.005

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	Crystal Structures of Cobalamin-Independent Methionine Synthase (MetE) from Streptococcus mutans: A Dynamic Zinc-Inversion Model
	Fu, Tian-Min ; Almqvist, Jonas ; Liang, Yu-He ; Li, Lanfen ; Huang, Yafei ; Su, Xiao-Dong
刊名	分子生物学杂志
	2011
关键词	MetE dynamic equilibrium methionine synthase homocysteine methyltetrahydrofolate ESCHERICHIA-COLI HOMOCYSTEINE ENZYMES METHYLTETRAHYDROFOLATE ADENOSYLMETHIONINE SITE
DOI	10.1016/j.jmb.2011.08.005
英文摘要	Cobalamin-independent methionine synthase (MetE) catalyzes the direct transfer of a methyl group from methyltetrahydrofolate to L-homocysteine to form methionine. Previous studies have shown that the MetE active site coordinates a zinc atom, which is thought to act as a Lewis acid and plays a role in the activation of thiol. Extended X-ray absorption fine structure studies and mutagenesis experiments identified the zinc-binding site in MetE from Escherichia coli. Further structural investigations of MetE from Thermotoga maritima lead to the proposition of two models: "induced fit" and "dynamic equilibrium", to account for the catalytic mechanisms of MetE. Here, we present crystal structures of oxidized and zinc-replete MetE from Streptococcus mutans at the physiological pH. The structures reveal that zinc is mobile in the active center and has the possibility to invert even in the absence of homocysteine. These structures provide evidence for the dynamic equilibrium model. (C) 2011 Elsevier Ltd. All rights; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000295496500012&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; SCI(E); PubMed; 7; ARTICLE; 4; 688-697; 412
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/193211]
专题	生命科学学院
推荐引用方式 GB/T 7714	Fu, Tian-Min,Almqvist, Jonas,Liang, Yu-He,et al. Crystal Structures of Cobalamin-Independent Methionine Synthase (MetE) from Streptococcus mutans: A Dynamic Zinc-Inversion Model[J]. 分子生物学杂志,2011.
APA	Fu, Tian-Min,Almqvist, Jonas,Liang, Yu-He,Li, Lanfen,Huang, Yafei,&Su, Xiao-Dong.(2011).Crystal Structures of Cobalamin-Independent Methionine Synthase (MetE) from Streptococcus mutans: A Dynamic Zinc-Inversion Model.分子生物学杂志.
MLA	Fu, Tian-Min,et al."Crystal Structures of Cobalamin-Independent Methionine Synthase (MetE) from Streptococcus mutans: A Dynamic Zinc-Inversion Model".分子生物学杂志 (2011).