Preliminary crystallographic analysis of two hypothetical   ribose-5-phosphate isomerases from Streptococcus mutans

doi:10.1107/S1744309112009761

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	Preliminary crystallographic analysis of two hypothetical ribose-5-phosphate isomerases from Streptococcus mutans
	Wang, Chen ; Fan, Xuexin ; Cao, Xiaofang ; Liu, Xiang ; Li, Lanfen ; Su, Xiaodong
刊名	acta crystallographica section f structural biology and crystallization communications
	2012
关键词	ESCHERICHIA-COLI D-RIBOSE-5-PHOSPHATE ISOMERASE
DOI	10.1107/S1744309112009761
英文摘要	Study of the enzymes from sugar metabolic pathways may provide a better understanding of the pathogenesis of the human oral pathogen Streptococcus mutans. Bioinformatics, biochemical and crystallization methods were used to characterize and understand the function of two putative ribose-5-phosphate isomerases: SMU1234 and SMU2142. The proteins were cloned and constructed with N-terminal His tags. Protein purification was performed by Ni2+-chelating and size-exclusion chromatography. The crystals of SUM1234 diffracted to 1.9 angstrom resolution and belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 48.97, b = 98.27, c = 101.09 angstrom, alpha = beta = gamma = 90 degrees. The optimized SMU2142 crystals diffracted to 2.7 angstrom resolution and belonged to space group P1, with unit-cell parameters a = 53.7, b = 54.1, c = 86.5 angstrom, alpha = 74.2, beta = 73.5, gamma = 83.7 degrees. Initial phasing of both proteins was attempted by molecular replacement; the structure of SMU1234 could easily be solved, but no useful results were obtained for SMU2142. Therefore, SeMet-labelled SMU2142 will be prepared for phasing.; Biochemical Research Methods; Biochemistry & Molecular Biology; Biophysics; Crystallography; SCI(E); PubMed; 0; ARTICLE; Pt 5; 565-568; 68
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/192140]
专题	生命科学学院
推荐引用方式 GB/T 7714	Wang, Chen,Fan, Xuexin,Cao, Xiaofang,et al. Preliminary crystallographic analysis of two hypothetical ribose-5-phosphate isomerases from Streptococcus mutans[J]. acta crystallographica section f structural biology and crystallization communications,2012.
APA	Wang, Chen,Fan, Xuexin,Cao, Xiaofang,Liu, Xiang,Li, Lanfen,&Su, Xiaodong.(2012).Preliminary crystallographic analysis of two hypothetical ribose-5-phosphate isomerases from Streptococcus mutans.acta crystallographica section f structural biology and crystallization communications.
MLA	Wang, Chen,et al."Preliminary crystallographic analysis of two hypothetical ribose-5-phosphate isomerases from Streptococcus mutans".acta crystallographica section f structural biology and crystallization communications (2012).