Structural and functional characterization of a novel alpha/beta   hydrolase from cariogenic pathogen Streptococcus mutans

doi:10.1002/prot.24418

CORC > 北京大学 > 生命科学学院

	Structural and functional characterization of a novel alpha/beta hydrolase from cariogenic pathogen Streptococcus mutans
	Wang, Zixi ; Li, Lanfen ; Su, Xiao-Dong
刊名	proteins structure function and bioinformatics
	2014
关键词	X-ray crystallography carboxylesterase catalytic triad Smu.1393c cap domain GENOMICS FOLD CONSERVATION DATABASE PROTEIN
DOI	10.1002/prot.24418
英文摘要	The protein Smu.1393c from Streptococcus mutans is annotated as a putative alpha/beta hydrolase, but it has low sequence identity to the structure-known alpha/beta hydrolases. Here we present the crystal structure of Smu.1393c at 2.0 angstrom resolution. Smu.1393c has a fully open alkaline substrate pocket, whose conformation is unique among other similar hydrolase structures. Three residues, Ser101, His251, and Glu125, were identified as the active center of Smu.1393c. By screening a series of artificial hydrolase substrates, we demonstrated Smu.1393c had low carboxylesterase activity towards short-chain carboxyl esters, which provided a clue for exploring the in vivo function of Smu.1393c. Proteins 2014; 82:695-700. (c) 2013 Wiley Periodicals, Inc.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000332306500016&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Biophysics; SCI(E); PubMed; 1; ARTICLE; xdsu@pku.edu.cn; 4; 695-700; 82
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/189912]
专题	生命科学学院
推荐引用方式 GB/T 7714	Wang, Zixi,Li, Lanfen,Su, Xiao-Dong. Structural and functional characterization of a novel alpha/beta hydrolase from cariogenic pathogen Streptococcus mutans[J]. proteins structure function and bioinformatics,2014.
APA	Wang, Zixi,Li, Lanfen,&Su, Xiao-Dong.(2014).Structural and functional characterization of a novel alpha/beta hydrolase from cariogenic pathogen Streptococcus mutans.proteins structure function and bioinformatics.
MLA	Wang, Zixi,et al."Structural and functional characterization of a novel alpha/beta hydrolase from cariogenic pathogen Streptococcus mutans".proteins structure function and bioinformatics (2014).