Convergence of Light and ABA Signaling on the ABI5 Promoter

doi:10.1371/journal.pgen.1004197

CORC > 北京大学 > 生命科学学院

	Convergence of Light and ABA Signaling on the ABI5 Promoter
	Xu, Dongqing ; Li, Jigang ; Gangappa, Sreeramaiah N. ; Hettiarachchi, Chamari ; Lin, Fang ; Andersson, Mats X. ; Jiang, Yan ; Deng, Xing Wang ; Holm, Magnus
刊名	plos genetics
	2014
关键词	B-BOX PROTEIN ARABIDOPSIS SEED DEVELOPMENT TRANSCRIPTION FACTOR HY5 ABSCISIC-ACID SYNTHESIS GENOME-WIDE ANALYSIS GENE-EXPRESSION UV-B PLANT-RESPONSES PHYTOCHROME GERMINATION
DOI	10.1371/journal.pgen.1004197
英文摘要	Author Summary Many factors such as light, phytohormone abscisic acid (ABA), etc., regulate multiple developmental processes throughout the plants' life cycle. Light promotes seed germination and ABA maintains seed dormancy. However, little is known about how light and ABA signaling pathways interact with each other. It was previously reported that Arabidopsis HY5, a well-known bZIP transcription factor involved in promoting seedling photomorphogenesis, is involved in ABA signaling by directly activating ABI5 expression. Here, we report that the B-box protein BBX21 negatively regulates ABI5 expression by interfering with HY5 binding to the ABI5 promoter. Interestingly, ABI5 was shown to directly bind to its own promoter and activate its expression, whereas BBX21 also negatively regulates this activity by interacting with ABI5. Together, our study shows that light and ABA signaling pathways converge on the ABI5 promoter, on which BBX21 acts as a negative regulator of ABI5 expression. Light is one of the most important environmental cues regulating multiple aspects of plant growth and development, and abscisic acid (ABA) is a plant hormone that plays important roles during many phases of the plant life cycle and in plants' responses to various environmental stresses. How plants integrate the external light signal with endogenous ABA pathway for better adaptation and survival remains poorly understood. Here, we show that BBX21 (also known as SALT TOLERANCE HOMOLOG 2), a B-box (BBX) protein previously shown to positively regulate seedling photomorphogenesis, is also involved in ABA signaling. Our genetic data show that BBX21 may act upstream of several ABA INSENSITIVE (ABI) genes and ELONGATED HYPOCOTYL 5 (HY5) in ABA control of seed germination. Previous studies showed that HY5 acts as a direct activator of ABI5 expression, and that BBX21 interacts with HY5. We further demonstrate that BBX21 negatively regulates ABI5 expression by interfering with HY5 binding to the ABI5 promoter. In addition, ABI5 was shown to directly activate its own expression, whereas BBX21 negatively regulates this activity by directly interacting with ABI5. Together, our study indicates that BBX21 coordinates with HY5 and ABI5 on the ABI5 promoter and that these transcriptional regulators work in concert to integrate light and ABA signaling in Arabidopsis thaliana.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000332021500042&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Genetics & Heredity; SCI(E); PubMed; 30; ARTICLE; xingwang.deng@yale.edu; 2; e1004197; 10
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/189418]
专题	生命科学学院
推荐引用方式 GB/T 7714	Xu, Dongqing,Li, Jigang,Gangappa, Sreeramaiah N.,et al. Convergence of Light and ABA Signaling on the ABI5 Promoter[J]. plos genetics,2014.
APA	Xu, Dongqing.,Li, Jigang.,Gangappa, Sreeramaiah N..,Hettiarachchi, Chamari.,Lin, Fang.,...&Holm, Magnus.(2014).Convergence of Light and ABA Signaling on the ABI5 Promoter.plos genetics.
MLA	Xu, Dongqing,et al."Convergence of Light and ABA Signaling on the ABI5 Promoter".plos genetics (2014).