Targeted Lipidomics Studies Reveal that Linolenic Acid Promotes Cotton Fiber Elongation by Activating Phosphatidylinositol and Phosphatidylinositol Monophosphate Biosynthesis

doi:10.1016/j.molp.2015.02.010

CORC > 北京大学 > 生命科学学院

	Targeted Lipidomics Studies Reveal that Linolenic Acid Promotes Cotton Fiber Elongation by Activating Phosphatidylinositol and Phosphatidylinositol Monophosphate Biosynthesis
	Liu Gao-Jun ; Xiao Guang-Hui ; Liu Ning-Jing ; Liu Dan ; Chen Pei-Shuang ; Qin Yong-Mei ; Zhu Yu-Xian
刊名	分子植物
	2015
关键词	Gossypium hirsutum,fiber elongation,glycerophospholipid profiling,mass spectrometry,phosphatidylinositol,phosphatidylinositol monophosphate
DOI	10.1016/j.molp.2015.02.010
英文摘要	The membrane lipids from fast-elongating wild-type cotton (Gossypium hirsutum) fibers at 10?days post-anthesis, wild-type ovules with fiber cells removed, and ovules from the fuzzless-lintless mutant harvested at the same age, were extracted, separated, and quantified. Fiber cells contained significantly higher amounts of phosphatidylinositol (PI) than both ovule samples with PI 34:3 being the most predominant species. The genes encoding fatty acid desaturases (Δ(15)GhFAD), PI synthase (PIS) and PI kinase (PIK) were expressed in a fiber-preferential manner. Further analysis of phosphatidylinositol monophosphate (PIP) indicated that elongating fibers contained four- to five-fold higher amounts of PIP 34:3 than the ovules. Exogenously applied linolenic acid (C18:3), soybean L-α-PI, and PIPs containing PIP 34:3 promoted significant fiber growth, whereas a liver PI lacking the C18:3 moiety, linoleic acid, and PIP 36:2 were completely ineffective. The growth inhibitory effects of carbenoxolone, 5-hydroxytryptamine, and wortmannin were reverted by C18:3, PI, or PIP, respectively, suggesting that PIP signaling is essential for fiber cell growth. Furthermore, cotton plants expressing virus-induced gene-silencing constructs that specifically suppressed GhΔ(15)FAD, GhPIS, or GhPIK expression, resulted in significantly short-fibered phenotypes. Our data provide the basis for in-depth studies on the roles of PI and PIP in mediating cotton fiber growth.Copyright ? 2015 The Author. Published by Elsevier Inc. All rights reserved.; SCI(E); PubMed; 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 0; ARTICLE; qinym@pku.edu.cn
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/188462]
专题	生命科学学院
推荐引用方式 GB/T 7714	Liu Gao-Jun,Xiao Guang-Hui,Liu Ning-Jing,et al. Targeted Lipidomics Studies Reveal that Linolenic Acid Promotes Cotton Fiber Elongation by Activating Phosphatidylinositol and Phosphatidylinositol Monophosphate Biosynthesis[J]. 分子植物,2015.
APA	Liu Gao-Jun.,Xiao Guang-Hui.,Liu Ning-Jing.,Liu Dan.,Chen Pei-Shuang.,...&Zhu Yu-Xian.(2015).Targeted Lipidomics Studies Reveal that Linolenic Acid Promotes Cotton Fiber Elongation by Activating Phosphatidylinositol and Phosphatidylinositol Monophosphate Biosynthesis.分子植物.
MLA	Liu Gao-Jun,et al."Targeted Lipidomics Studies Reveal that Linolenic Acid Promotes Cotton Fiber Elongation by Activating Phosphatidylinositol and Phosphatidylinositol Monophosphate Biosynthesis".分子植物 (2015).