High-throughput screens in mammalian cells using the CRISPR-Cas9 system

doi:10.1111/febs.13251

CORC > 北京大学 > 生命科学学院

	High-throughput screens in mammalian cells using the CRISPR-Cas9 system
	Peng Jingyu ; Zhou Yuexin ; Zhu Shiyou ; Wei Wensheng
刊名	the febs journal
	2015
关键词	CRISPR-Cas9 system,high-throughput,knockout,screening,sgRNA
DOI	10.1111/febs.13251
英文摘要	As a powerful genome-editing tool, the clustered regularly interspaced short palindromic repeats (CRISPR)-clustered regularly interspaced short palindromic repeats-associated protein 9 (Cas9) system has been quickly developed into a large-scale function-based screening strategy in mammalian cells. This new type of genetic library is constructed through the lentiviral delivery of single-guide RNA collections that direct Cas9 or inactive dead Cas9 fused with effectors to interrogate gene function or regulate gene transcription in targeted cells. Compared with RNA interference screening, the CRISPR-Cas9 system demonstrates much higher levels of effectiveness and reliability with respect to both loss-of-function and gain-of-function screening. Unlike the RNA interference strategy, a CRISPR-Cas9 library can target both protein-coding sequences and regulatory elements, including promoters, enhancers and elements transcribing microRNAs and long noncoding RNAs. This powerful genetic tool will undoubtedly accelerate the mechanistic discovery of various biological processes. In this mini review, we summarize the general procedure of CRISPR-Cas9 library mediated functional screening, system optimization strategies and applications of this new genetic toolkit.? 2015 FEBS.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000355664000003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); PubMed; 18; REVIEW; wswei@pku.edu.cn
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/188461]
专题	生命科学学院
推荐引用方式 GB/T 7714	Peng Jingyu,Zhou Yuexin,Zhu Shiyou,et al. High-throughput screens in mammalian cells using the CRISPR-Cas9 system[J]. the febs journal,2015.
APA	Peng Jingyu,Zhou Yuexin,Zhu Shiyou,&Wei Wensheng.(2015).High-throughput screens in mammalian cells using the CRISPR-Cas9 system.the febs journal.
MLA	Peng Jingyu,et al."High-throughput screens in mammalian cells using the CRISPR-Cas9 system".the febs journal (2015).