Brief Reports: TRPM7 Senses Mechanical Stimulation Inducing Osteogenesis   in Human Bone Marrow Mesenchymal Stem Cells

doi:10.1002/stem.1858

CORC > 北京大学 > 生命科学学院

	Brief Reports: TRPM7 Senses Mechanical Stimulation Inducing Osteogenesis in Human Bone Marrow Mesenchymal Stem Cells
	Xiao, E. ; Yang, H. Q. ; Gan, Ye-Hua ; Duan, Deng-Hui ; He, Lin-Hai ; Guo, Yunbo ; Wang, S. Q. ; Zhang, Yi
刊名	stem cells
	2015
关键词	Marrow stromal stem cells Mesenchymal stem cells Calcium flux Differentiation and mechanosensation OSTEOBLAST DIFFERENTIATION FLUID-FLOW CHANNELS ACTIVATION KINASE GENE MECHANOSENSATION PROLIFERATION STRETCH
DOI	10.1002/stem.1858
英文摘要	Mesenchymal stem cells (MSCs) are multipotential stem cells residing in the bone marrow. Several studies have shown that mechanical stimulation modulates MSC differentiation through mobilization of second messengers, but the mechanism of mechanotransduction remains poorly understood. In this study, using fluorescence and laser confocal microcopy as well as patch-clamp techniques, we identified the transient receptor potential melastatin type 7 (TRPM7) channel as the key channel involved in mechanotransduction in bone marrow MSCs. TRPM7 knockdown completely abolished the pressure-induced cytosolic Ca2+ increase and pressure-induced osteogenesis. TRPM7 directly sensed membrane tension, independent of the cytoplasm and the integrity of cytoskeleton. Ca2+ influx through TRPM7 further triggered Ca2+ release from the inositol trisphosphate receptor type 2 on the endoplasmic reticulum and promoted NFATc1 nuclear localization and osteogenesis. These results identified a central role of TRPM7 in MSC mechanical stimulation-induced osteogenesis.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000348211300026&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Cell & Tissue Engineering; Biotechnology & Applied Microbiology; Oncology; Cell Biology; Hematology; SCI(E); PubMed; 3; ARTICLE; wsq@pku.edu.cn; zhangyi2000@263.net; 2; 615-621; 33
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/163020]
专题	生命科学学院
推荐引用方式 GB/T 7714	Xiao, E.,Yang, H. Q.,Gan, Ye-Hua,et al. Brief Reports: TRPM7 Senses Mechanical Stimulation Inducing Osteogenesis in Human Bone Marrow Mesenchymal Stem Cells[J]. stem cells,2015.
APA	Xiao, E..,Yang, H. Q..,Gan, Ye-Hua.,Duan, Deng-Hui.,He, Lin-Hai.,...&Zhang, Yi.(2015).Brief Reports: TRPM7 Senses Mechanical Stimulation Inducing Osteogenesis in Human Bone Marrow Mesenchymal Stem Cells.stem cells.
MLA	Xiao, E.,et al."Brief Reports: TRPM7 Senses Mechanical Stimulation Inducing Osteogenesis in Human Bone Marrow Mesenchymal Stem Cells".stem cells (2015).