Isolation and characterization of a gene encoding cinnamate   4-hydroxylase from Parthenocissus henryana

doi:10.1007/s11033-008-9357-6

CORC > 北京大学 > 生命科学学院

	Isolation and characterization of a gene encoding cinnamate 4-hydroxylase from Parthenocissus henryana
	Liu, Shujun ; Hu, Yuanlei ; Wang, Xiaoli ; Han, Li ; Song, Songquan ; Cheng, Hongyan ; Lin, Zhongping
刊名	分子生物学报告
	2009
关键词	Cinnamate 4-hydroxylase Parthenocissus henryana Phenylpropanoid pathway Promoter analysis ACID 4-HYDROXYLASE PLANT CYTOCHROME-P450 MOLECULAR-CLONING STILBENE SYNTHASE ACTIVE-SITE MYB GENE EXPRESSION ARABIDOPSIS TRANSCRIPTION BIOSYNTHESIS
DOI	10.1007/s11033-008-9357-6
英文摘要	Cinnamate 4-hydroxylase (C4H, EC 1.14.13.11) plays an important role in the phenylpropanoid pathway, which produces many economically important secondary metabolites. A gene coding for C4H, designated as PhC4H (GenBank accession no. DQ211885) was isolated from Parthenocissus henryana. The full-length PhC4H cDNA is 1,747 bp long with a 1,518-bp open reading frame encoding a protein of 505 amino acids, a 40-bp 5' non-coding region and a 189-bp 3'-untranslated region. Secondary structure of the deduced PhC4H protein consists of 41.78% alpha helix, 15.64% extended strand and 42.57% random coil. The genomic DNA of PhC4H is 2,895 bp long and contains two introns; intron I is 205-bp and intron II is 1,172-bp (GenBank accession no. EU440734). DNA gel blot analysis revealed that there might be a single copy of PhC4H in Parthenocissus henryana genome. By using anchored PCR, a 963-bp promoter sequence was isolated and it contains many responsive elements conserved in the upstream region of PAL, C4H and 4CL including the P-, A-, L- and H-boxes.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000266454500056&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; SCI(E); PubMed; 4; ARTICLE; 6; 1605-1610; 36
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/162590]
专题	生命科学学院
推荐引用方式 GB/T 7714	Liu, Shujun,Hu, Yuanlei,Wang, Xiaoli,et al. Isolation and characterization of a gene encoding cinnamate 4-hydroxylase from Parthenocissus henryana[J]. 分子生物学报告,2009.
APA	Liu, Shujun.,Hu, Yuanlei.,Wang, Xiaoli.,Han, Li.,Song, Songquan.,...&Lin, Zhongping.(2009).Isolation and characterization of a gene encoding cinnamate 4-hydroxylase from Parthenocissus henryana.分子生物学报告.
MLA	Liu, Shujun,et al."Isolation and characterization of a gene encoding cinnamate 4-hydroxylase from Parthenocissus henryana".分子生物学报告 (2009).