The modified castor bean catalase intron is incompletely spliced in   tobacco and Arabidopsis

doi:10.1016/j.plantsci.2011.05.006

CORC > 北京大学 > 生命科学学院

	The modified castor bean catalase intron is incompletely spliced in tobacco and Arabidopsis
	Ma, Hong ; Lin, Zhongping ; Hu, Yuanlei
刊名	plant science
	2011
关键词	Castor bean catalase intron Cre-loxP recombination system Incomplete splicing Gus gene Allele-specific PCR SITE-SPECIFIC RECOMBINATION TRANSGENIC PLANTS GENE-EXPRESSION CRE RECOMBINASE MESSENGER-RNA MEDIATED ENHANCEMENT REPORTER GENE DNA EXCISION PROTEIN
DOI	10.1016/j.plantsci.2011.05.006
英文摘要	In an attempt to insert the modified castor bean catalase intron (mCBC intron) into the coding sequence of the Cre recombinase gene, we found that the mCBC intron was not completely spliced from the resulting iCre gene in tobacco and Arabidopsis. Sequencing and allele-specific PCR analyses indicated that six nucleotides (UUACAG) at the 3' terminus of the mCBC intron were retained in the mature mRNA of the iCre gene. Moreover, the mCBC intron was incompletely spliced from the Gus gene in pCAMBIA vectors. A mutational analysis of the mCBC intron demonstrated that the incomplete splicing was due to an artificial 3' splice site introduced by the insertion of an adenine, which created a TAG (stop) codon near the 3' splice site of the original CBC intron. Deletion of the inserted adenine or the six nucleotides that were retained from the mCBC intron led to the complete removal of the intron from the resulting iCre2 and iCre3 genes. Thus, in this study, we not only characterized the incomplete splicing event of the mCBC intron in tobacco and Arabidopsis, but also reported the construction of two intron-containing Cre recombinase genes that are useful for plant biotechnology applications. (C) 2011 Elsevier Ireland Ltd. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000292673200015&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Plant Sciences; SCI(E); PubMed; 1; ARTICLE; 2; 188-194; 181
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/162525]
专题	生命科学学院
推荐引用方式 GB/T 7714	Ma, Hong,Lin, Zhongping,Hu, Yuanlei. The modified castor bean catalase intron is incompletely spliced in tobacco and Arabidopsis[J]. plant science,2011.
APA	Ma, Hong,Lin, Zhongping,&Hu, Yuanlei.(2011).The modified castor bean catalase intron is incompletely spliced in tobacco and Arabidopsis.plant science.
MLA	Ma, Hong,et al."The modified castor bean catalase intron is incompletely spliced in tobacco and Arabidopsis".plant science (2011).