MilQuant: A free, generic software tool for isobaric tagging-based   quantitation

doi:10.1016/j.jprot.2012.06.028

CORC > 北京大学 > 生命科学学院

	MilQuant: A free, generic software tool for isobaric tagging-based quantitation
	Zou, Xiao ; Zhao, Minzhi ; Shen, Hongyan ; Zhao, Xuyang ; Tong, Yuanpeng ; Wang, Qingsong ; Wei, Shicheng ; Ji, Jianguo
刊名	journal of proteomics
	2012
关键词	Isobaric tagging iTRAQ MilQuant Public software Quantitative proteomics TMT PROTEIN QUANTIFICATION PEPTIDE QUANTIFICATION LTQ ORBITRAP PROTEOMICS ITRAQ PIPELINE
DOI	10.1016/j.jprot.2012.06.028
英文摘要	Isobaric tagging techniques such as iTRAQ and TMT are widely used in quantitative proteomics and especially useful for samples that demand in vitro labeling. Due to diversity in choices of MS acquisition approaches, identification algorithms, and relative abundance deduction strategies, researchers are faced with a plethora of possibilities when it comes to data analysis. However, the lack of generic and flexible software tool often makes it cumbersome for researchers to perform the analysis entirely as desired. In this paper, we present MilQuant, mzXML-based isobaric labeling quantitator, a pipeline of freely available programs that supports native acquisition files produced by all mass spectrometer types and collection approaches currently used in isobaric tagging based MS data collection. Moreover, aside from effective normalization and abundance ratio deduction algorithms, MilQuant exports various intermediate results along each step of the pipeline, making it easy for researchers to customize the analysis. The functionality of MilQuant was demonstrated by four distinct datasets from different laboratories. The compatibility and extendibility of MilQuant makes it a generic and flexible tool that can serve as a full solution to data analysis of isobaric tagging-based quantitation. (C) 2012 Elsevier B.V. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000309493400030&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemical Research Methods; SCI(E); PubMed; 2; ARTICLE; 17; 5516-5522; 75
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/162500]
专题	生命科学学院
推荐引用方式 GB/T 7714	Zou, Xiao,Zhao, Minzhi,Shen, Hongyan,et al. MilQuant: A free, generic software tool for isobaric tagging-based quantitation[J]. journal of proteomics,2012.
APA	Zou, Xiao.,Zhao, Minzhi.,Shen, Hongyan.,Zhao, Xuyang.,Tong, Yuanpeng.,...&Ji, Jianguo.(2012).MilQuant: A free, generic software tool for isobaric tagging-based quantitation.journal of proteomics.
MLA	Zou, Xiao,et al."MilQuant: A free, generic software tool for isobaric tagging-based quantitation".journal of proteomics (2012).