Heat shock protein 70 gene family in the Glanville fritillary butterfly   and their response to thermal stress

doi:10.1016/j.gene.2014.11.043

CORC > 北京大学 > 生命科学学院

	Heat shock protein 70 gene family in the Glanville fritillary butterfly and their response to thermal stress
	Luo, Shiqi ; Ahola, Virpi ; Shu, Chang ; Xu, Chongren ; Wang, Rongjiang
刊名	gene
	2015
关键词	Hsp70 Glanville fritillary Thermal stress Acclimation HEAT-SHOCK PROTEINS ADULT DROSOPHILA-MELANOGASTER HSP70 MULTIGENE FAMILY ENVIRONMENTAL-TEMPERATURE QUANTITATIVE PCR METABOLIC-RATE CLIMATE-CHANGE LIFE-HISTORY COLD SHOCK EXPRESSION
DOI	10.1016/j.gene.2014.11.043
英文摘要	Temperature variation in the environment is a great challenge to organisms. Induction of heat shock protein 70 (HSP70) is a common genetic mechanism to cope with thermal stress. The Glanville fritillary butterfly (Melitaea cinxia) is a model species in population and evolutionary biology, and its behavior and life history are greatly influenced by ambient temperature. We cloned and sequenced the full coding sequences of seven hsp70 genes from the Glanville fritillary. Of those genes, McHsc70-1 and McHsc70-2 were identified as heat shock cognate 70 (hsc70), of which the latter located in endoplasmic reticulum. We analyzed the expression patterns of different hsp70s under various thermal stresses using quantitative PCR. Heat shock at 40 degrees C for 2 h induced high expression of McHsp70-1, McHsp70-2 and McHsc70-2. Only McHsc70-2 had a small increase after cold shock at 0 degrees C for 2 h. Acclimation at 35 degrees C for three days before heat shock reduced expression of McHsp70 after heat shock. The maximum mRNA level of McHsp70s was reached in the first 2 h after the heat shock. This study uncovers the complexity of the hsp70 system, and provides the valuable information for further temperature-related research in the Glanville fritillary butterfly. (C) 2014 Elsevier B.V. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000349589800008&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Genetics & Heredity; SCI(E); PubMed; 2; ARTICLE; xchr@pku.edu.cn; rjwang@pku.edu.cn; 2; 132-141; 556
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/162426]
专题	生命科学学院
推荐引用方式 GB/T 7714	Luo, Shiqi,Ahola, Virpi,Shu, Chang,et al. Heat shock protein 70 gene family in the Glanville fritillary butterfly and their response to thermal stress[J]. gene,2015.
APA	Luo, Shiqi,Ahola, Virpi,Shu, Chang,Xu, Chongren,&Wang, Rongjiang.(2015).Heat shock protein 70 gene family in the Glanville fritillary butterfly and their response to thermal stress.gene.
MLA	Luo, Shiqi,et al."Heat shock protein 70 gene family in the Glanville fritillary butterfly and their response to thermal stress".gene (2015).