烟草果胶甲基酯酶 (PME)基因的克隆及功能分析(英文)

doi:10.3321/j.issn:1000-3282.2004.07.012

CORC > 北京大学 > 生命科学学院

	烟草果胶甲基酯酶 (PME)基因的克隆及功能分析(英文); Cloning and functional analysis of tobacco pectin methylesterase
	李春波 ; 高锋 ; 钟永旺 ; 魏春红 ; 李毅
刊名	生物化学与生物物理进展
	2004
关键词	果胶甲基酯酶序列分析酵母双杂交运动蛋白 pectin methylesterase sequence analysis yeast two-hybrid system movement protein
DOI	10.3321/j.issn:1000-3282.2004.07.012
英文摘要	为了研究果胶甲基酯酶 (pectinmethyl esterase ,PME) (EC 3.1.1.11)与植物病毒运动蛋白之间的相互作用 ,应用RT PCR方法从烟草 (Nicotianabenthamiana)中克隆了PME基因 ,并测定了全序列 (GenBank登录号AY2 3896 8) .序列分析显示该基因由两个保守的结构域组成 (PMEI和pectinesterase) .DNA印迹结果表明 ,该基因在基因组中存在多个拷贝 ,蛋白质印迹表明 ,植物总蛋白中存在两种形式的PME蛋白 ,但RNA印迹结果显示 ,在烟草细胞中只检测到全长的PME转录产物 .酵母双杂交结果表明 ,PME与水...; In order to study the interactions between plant virus movement protein and pectin methylesterase (PME), PME gene from tobacco genome was cloned using RT-PCR method and the sequence was determined ( GenBank accession No. AY238968). Sequence analysis showed that there were two conserved domains in PME protein: PMEI and pectinesterase. Multiple copies of PME gene were detected in tobacco genome through Southern blot analysis. Western blot result indicated that two types of PME protein existed in tobacco. But Northern hybridization detected only a full length transcript of PME, which further identified that there was post-translational process. Yeast two-hybrid result demonstrated that PME could not interact with the identified movement protein of rice dwarf virus (RDV), Pns6, but does interact with Pns11, a nucleic acid binding protein of RDV. It implies Pns1 I may participate in the movement of RDV.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000222914400012&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; SCI(E); 中文核心期刊要目总览(PKU); 中国科技核心期刊(ISTIC); 中国科学引文数据库(CSCD); 0; 07; 643-649; 31
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/46702]
专题	生命科学学院
推荐引用方式 GB/T 7714	李春波,高锋,钟永旺,等. 烟草果胶甲基酯酶 (PME)基因的克隆及功能分析(英文), Cloning and functional analysis of tobacco pectin methylesterase[J]. 生物化学与生物物理进展,2004.
APA	李春波,高锋,钟永旺,魏春红,&李毅.(2004).烟草果胶甲基酯酶 (PME)基因的克隆及功能分析(英文).生物化学与生物物理进展.
MLA	李春波,et al."烟草果胶甲基酯酶 (PME)基因的克隆及功能分析(英文)".生物化学与生物物理进展 (2004).