Encapsulation of RNA by negatively charged human serum albumin via   physical interactions

doi:10.1007/s11426-016-0094-8

CORC > 北京大学 > 化学与分子工程学院

	Encapsulation of RNA by negatively charged human serum albumin via physical interactions
	Wen, Hao ; Yin, Yudan ; Huang, Chao ; Pan, Wei ; Liang, Dehai
刊名	SCIENCE CHINA-CHEMISTRY
	2017
关键词	biopolymers complexation electrostatic interaction nanoparticle drug delivery BLOCK-COPOLYMER MICELLES DRUG-DELIVERY HSA-NANOPARTICLES HEAT DENATURATION GENE DELIVERY MICROSPHERES SYSTEMS DESIGN TUMORS
DOI	10.1007/s11426-016-0094-8
英文摘要	Human serum albumin (HSA) is the most abundant plasma protein and has an inherent ability to target tumor cells. It is an excellent candidate for drug delivery. However, HSA cannot form complex with DNA or RNA, because it is negatively charged under physiological conditions. In this work, we reported a simple method to prepare HSA/RNA nanoparticles mainly by physical interaction. Firstly, the solution pH is adjusted to 4.0, under which condition HSA is positively charged. It forms complex with RNA via electrostatic interaction. The solution is then heated at 75 oC for 15 min to stabilize the structure and the size of the formed complex. The HSA/RNA nanoparticle prepared by this method has a diameter about 110 nm and a narrow distribution. It is also stable for days under physiological conditions. Cellular essays demonstrate that these particles exhibit a high cellular uptake efficiency and non-toxicity to HeLa cells.; National Natural Science Foundation of China [21574002]; SCI(E); 中国科学引文数据库(CSCD); ARTICLE; 1; 130-135; 60
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/476449]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Wen, Hao,Yin, Yudan,Huang, Chao,et al. Encapsulation of RNA by negatively charged human serum albumin via physical interactions[J]. SCIENCE CHINA-CHEMISTRY,2017.
APA	Wen, Hao,Yin, Yudan,Huang, Chao,Pan, Wei,&Liang, Dehai.(2017).Encapsulation of RNA by negatively charged human serum albumin via physical interactions.SCIENCE CHINA-CHEMISTRY.
MLA	Wen, Hao,et al."Encapsulation of RNA by negatively charged human serum albumin via physical interactions".SCIENCE CHINA-CHEMISTRY (2017).