DNA Dendrimer-Streptavidin Nanocomplex: an Efficient Signal Amplifier   for Construction of Biosensing Platforms

doi:10.1021/acs.analchem.7b01551

CORC > 北京大学 > 化学与分子工程学院

	DNA Dendrimer-Streptavidin Nanocomplex: an Efficient Signal Amplifier for Construction of Biosensing Platforms
	Zhao, Yan ; Hu, Shichao ; Wang, Huaming ; Yu, Kaiwen ; Guan, Yan ; Liu, Xiaoyun ; Li, Na ; Liu, Feng
刊名	ANALYTICAL CHEMISTRY
	2017
关键词	QUARTZ-CRYSTAL MICROBALANCE HYBRIDIZATION CHAIN-REACTION NUCLEIC-ACIDS CELL-SURFACE CANCER-CELLS AMPLIFICATION NANOSTRUCTURE NANOPARTICLE STRATEGY MUTATION
DOI	10.1021/acs.analchem.7b01551
英文摘要	We develop a DNA dendrimer-streptavidin (SA) nanocomplex as a novel signal amplifier to create biosensing platforms for disease-related species. The DNA dendrimer-SA nanocomplex is fabricated by cross-linking the nonlinear hybridization chain reaction based DNA dendrimer with the SA-coupled linker DNA and possesses multiple sticky ends, a high molecular weight, and a hyperbranched nanostructure with large numbers of DNA duplexes. Taking advantage of the DNA dendrimer-SA nanocomplex and a label-free quartz crystal microbalance (QCM) technology, we first construct a mass-sensitive QCM biosensing platform for nucleic acids, which displays high selectivity and sensitivity, with a detection limit of 0.062 nM KRAS gene fragment. Then we present a fluorescent sensing strategy toward HeLa cells by functionalizing the DNA dendrimer-SA nanocomplex using the sgc8 aptamer and the SYBR Green I intercalating dye. The spiked recoveries of targets in physiological media are greater than 90%, demonstrating potential application of created biosensing platforms in clinical diagnosis. This work expands the rule set of designing DNA nanomaterials for development of biosensing strategies, and provides universal platforms for detecting disease-related species through simply altering the related capture and reporter DNA sequences.; National Natural Science Foundation of China [21675005, 21535006, 21275013]; SCI(E); ARTICLE; 12; 6907-6914; 89
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/472739]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Zhao, Yan,Hu, Shichao,Wang, Huaming,et al. DNA Dendrimer-Streptavidin Nanocomplex: an Efficient Signal Amplifier for Construction of Biosensing Platforms[J]. ANALYTICAL CHEMISTRY,2017.
APA	Zhao, Yan.,Hu, Shichao.,Wang, Huaming.,Yu, Kaiwen.,Guan, Yan.,...&Liu, Feng.(2017).DNA Dendrimer-Streptavidin Nanocomplex: an Efficient Signal Amplifier for Construction of Biosensing Platforms.ANALYTICAL CHEMISTRY.
MLA	Zhao, Yan,et al."DNA Dendrimer-Streptavidin Nanocomplex: an Efficient Signal Amplifier for Construction of Biosensing Platforms".ANALYTICAL CHEMISTRY (2017).