Combination of antibody-coated, physical-based microfluidic chip with   wave-shaped arrays for isolating circulating tumor cells

doi:10.1007/s10544-017-0202-3

CORC > 北京大学 > 化学与分子工程学院

	Combination of antibody-coated, physical-based microfluidic chip with wave-shaped arrays for isolating circulating tumor cells
	Chen, Hongmei ; Cao, Baoshan ; Chen, Hongda ; Lin, Yu-Sheng ; Zhang, Jingjing
刊名	BIOMEDICAL MICRODEVICES
	2017
关键词	Circulating tumor cells (CTCs) Epithelial cell adhension molecule (EpCAM) Epithelial-to-mesenchymal transition (EMT) CANCER-CELLS WHOLE-BLOOD DEVICE CAPTURE SIZE ENUMERATION TECHNOLOGY ENRICHMENT SEPARATION RELEVANCE
DOI	10.1007/s10544-017-0202-3
英文摘要	Circulating tumor cells (CTCs) are found in the peripheral blood of patients with metastatic cancers, which have critical significance in cancer prognosis and diagnostics. Enumeration is significantly valuable since number of CTCs is strongly correlated to severity of disease. This article is proposed and demonstrated an antibody-coated, size-based microfluidic chip with wave-shaped arrays could efficiently capture CTCs combining two separation methods of both size-and deformability-based and affinity-based segregation. Utilizing immunocapture of capture chemistry of Epithelial Cell Adhension Molecule (EpCAM), tumor cells could be captured by narrow gaps or have a friction with microposts edges to realize both immune-affinity and size capture. This wave-shaped layout of microfluidic chip with varying gaps between adjacent circular microposts can generate perpendicular velocities to the fluidic direction. This oriented fluidic direction will carry cells to next smaller neighboring gap and then be captured gradually. The experiment results indicate capture efficiency is similar to 90% and viability is similar to 95% after extracted and cultured 3 days. Furthermore, this chip has been validated for whole blood with cancer cell lines and mimic patient blood. This study demonstrates feasibility using our microfluidic chip for CTCs research, monitoring cancer progress and evaluating therapeutic treatment.; Major State Basic Research Development Program of China (973 Program) [2011CB933102]; National Natural Science Foundation of China (Key Program) [61335010]; Key Research Program of Chinese Academy of Sciences [KJZD-EW-TZ-L03-6]; Postdoctoral Research Funding [2014 M550794]; SCI(E); ARTICLE; 3; 19
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/471066]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Chen, Hongmei,Cao, Baoshan,Chen, Hongda,et al. Combination of antibody-coated, physical-based microfluidic chip with wave-shaped arrays for isolating circulating tumor cells[J]. BIOMEDICAL MICRODEVICES,2017.
APA	Chen, Hongmei,Cao, Baoshan,Chen, Hongda,Lin, Yu-Sheng,&Zhang, Jingjing.(2017).Combination of antibody-coated, physical-based microfluidic chip with wave-shaped arrays for isolating circulating tumor cells.BIOMEDICAL MICRODEVICES.
MLA	Chen, Hongmei,et al."Combination of antibody-coated, physical-based microfluidic chip with wave-shaped arrays for isolating circulating tumor cells".BIOMEDICAL MICRODEVICES (2017).