Chiral nanoprobes for targeting and long-term imaging of the Golgi   apparatus

doi:10.1039/c7sc01316g

CORC > 北京大学 > 化学与分子工程学院

	Chiral nanoprobes for targeting and long-term imaging of the Golgi apparatus
	Li, Rong Sheng ; Gao, Peng Fei ; Zhang, Hong Zhi ; Zheng, Lin Ling ; Li, Chun Mei ; Wang, Jian ; Li, Yuan Fang ; Liu, Feng ; Li, Na ; Huang, Cheng Zhi
刊名	CHEMICAL SCIENCE
	2017
关键词	CARBON DOTS CELLS PROTEIN CYSTEINE COMPLEX DOMAIN PROBE
DOI	10.1039/c7sc01316g
英文摘要	The Golgi apparatus is an essential subcellular organelle. Targeting and monitoring the Golgi change at the single-cell level over a long time scale are critical but are challenges that have not yet been tackled. Inspired by the precise Golgi positioning ability of galactosyltransferase and protein kinase D, due to their cysteine residues, we developed a method for long-term Golgi imaging. Fluorescent molecules, carbon quantum dots (CQDs) and silica nanoparticles could target the Golgi when they are modified with L-cysteine. L-Cysteine-rich chiral carbon quantum dots (LC-CQDs), which have the benefits of a high Golgi specificity from L-cysteine and excellent photostability and biocompatibility from the CQDs, are proven to be highly suitable for long-term in situ imaging of the Golgi. Investigation of the mechanism showed that free thiol groups and the L-type stereo configuration of LC-CQDs are essential for specific targeting of the Golgi. With the aid of the as-prepared LC-CQDs, the dynamic changes of the Golgi in the early stage of viral infection were visualized. The Golgi targeting and imaging strategy used in this work is beneficial for Golgi-targeted drug delivery and early diagnosis and therapy of Golgi diseases.; Natural Science Foundation Project of China [21535006, 21405123]; Chongqing Science & Technology Commission [cstc2014jcyjA50006]; Fundamental Research Funds for the Central Universities [XDJK2016B039]; National Basic Research Program of China (973 Program) [2011CB933600]; SCI(E); ARTICLE; 10; 6829-6835; 8
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/470619]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Li, Rong Sheng,Gao, Peng Fei,Zhang, Hong Zhi,et al. Chiral nanoprobes for targeting and long-term imaging of the Golgi apparatus[J]. CHEMICAL SCIENCE,2017.
APA	Li, Rong Sheng.,Gao, Peng Fei.,Zhang, Hong Zhi.,Zheng, Lin Ling.,Li, Chun Mei.,...&Huang, Cheng Zhi.(2017).Chiral nanoprobes for targeting and long-term imaging of the Golgi apparatus.CHEMICAL SCIENCE.
MLA	Li, Rong Sheng,et al."Chiral nanoprobes for targeting and long-term imaging of the Golgi apparatus".CHEMICAL SCIENCE (2017).