Nitrilase-Activatable Noncanonical Amino Acid Precursors for   Cell-Selective Metabolic Labeling of Proteomes

doi:10.1021/acschembio.6b00765

CORC > 北京大学 > 化学与分子工程学院

	Nitrilase-Activatable Noncanonical Amino Acid Precursors for Cell-Selective Metabolic Labeling of Proteomes
	Li, Zefan ; Zhu, Yuntao ; Sun, Yuting ; Qin, Ke ; Liu, Weibing ; Zhou, Wen ; Chen, Xing
刊名	ACS CHEMICAL BIOLOGY
	2016
关键词	NEWLY SYNTHESIZED PROTEINS MAMMALIAN-CELLS IN-VIVO IDENTIFICATION VISUALIZATION LIGATION AZIDES
DOI	10.1021/acschembio.6b00765
英文摘要	Cell-selective protein metabolic labeling is of great interest for studying cell cell communications and tissue homeostasis. We herein describe a nitrilase-activatable non canonical amino acid tagging (NANCAT) strategy that exploits an exogenous nitrilase to enzymatically convert the nitrite-substituted precursors to their corresponding noncanonical amino acids (ncAAs), L-azidohomoalanine (Aha) or homopropargylglycine (Hpg), in living cells. Only cells expressing the nitrilase can generate Aha or Hpg in cellulo and metabolically incorporate them into the nascent proteins. Subsequent click labeling of the azide- or alkyne-incorporated proteins with fluorescent probes or with affinity tags enables visualization and proteomic profiling of nascent proteomes, respectively. We have demonstrated that NANCAT can serve as a versatile strategy for cell-selective labeling of proteomes in both bacterial and mammalian cells.; National Natural Science Foundation of China [21425204]; National Key Research and Development Projects [2016YFA0501500]; SCI(E); PubMed; ARTICLE; xingchen@pku.edu.cn; 12; 3273-3277; 11
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/457153]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Li, Zefan,Zhu, Yuntao,Sun, Yuting,et al. Nitrilase-Activatable Noncanonical Amino Acid Precursors for Cell-Selective Metabolic Labeling of Proteomes[J]. ACS CHEMICAL BIOLOGY,2016.
APA	Li, Zefan.,Zhu, Yuntao.,Sun, Yuting.,Qin, Ke.,Liu, Weibing.,...&Chen, Xing.(2016).Nitrilase-Activatable Noncanonical Amino Acid Precursors for Cell-Selective Metabolic Labeling of Proteomes.ACS CHEMICAL BIOLOGY.
MLA	Li, Zefan,et al."Nitrilase-Activatable Noncanonical Amino Acid Precursors for Cell-Selective Metabolic Labeling of Proteomes".ACS CHEMICAL BIOLOGY (2016).