Nonamplification Sandwich Assay Platform for Sensitive Nucleic Acid   Detection Based on AuNPs Enumeration with the Dark-Field Microscope

doi:10.1021/acs.analchem.6b00535

CORC > 北京大学 > 化学与分子工程学院

	Nonamplification Sandwich Assay Platform for Sensitive Nucleic Acid Detection Based on AuNPs Enumeration with the Dark-Field Microscope
	Li, Tian ; Xu, Xiao ; Zhang, Guoqing ; Lin, Ruoyun ; Chen, Yang ; Li, Chenxi ; Liu, Feng ; Li, Na
刊名	ANALYTICAL CHEMISTRY
	2016
关键词	SINGLE PLASMONIC NANOPARTICLES LIGATION CHAIN-REACTION GOLD NANOPARTICLES ULTRASENSITIVE DETECTION MESSENGER-RNA LIVING CELLS SCATTERING PROPERTIES HOMOGENEOUS DETECTION METAL NANOPARTICLES DNA HYBRIDIZATION
DOI	10.1021/acs.analchem.6b00535
英文摘要	A simple and efficient assay platform with high sensitivity, convenient implementation, and moderate cost in reagents and instrumentation is most appropriate for routine applications. On the basis of the gold nanoparticle (AuNP) enumeration signal readout mode established in our laboratory, we have developed a nonamplification sandwich assay for nucleic acid detection with the 3 fM limit of detection for a sequence related to Alzheimer's disease. This AuNP counting based method takes advantages of the distinctive and strong localized surface plasmon resonance light scattering with the dark-field microscope and magnetic separation. It is shown that the presence of 20 nM random DNA sequence or calf thymus DNA with a mass up to 10(6)-fold of the targets do not significantly interfere with the assay signal. The spike recoveries of Hela cell lysate sample at 109.3% for 20 pM target and 110.5% for 100 pM target indicate the potential of this proposed method in practical sample applications. This nonamplification sandwich assay platform in principle is applicable to other assays such as the immunoassay and thus would be expected to find a breadth of applications that can make the best use of the simplicity and sensitivity.; National Natural Science Foundation of China [21475004, 21275011, 21535006, 21505125]; SCI(E); ARTICLE; lina@pku.edu.cn; 8; 4188-4191; 88
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/438284]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Li, Tian,Xu, Xiao,Zhang, Guoqing,et al. Nonamplification Sandwich Assay Platform for Sensitive Nucleic Acid Detection Based on AuNPs Enumeration with the Dark-Field Microscope[J]. ANALYTICAL CHEMISTRY,2016.
APA	Li, Tian.,Xu, Xiao.,Zhang, Guoqing.,Lin, Ruoyun.,Chen, Yang.,...&Li, Na.(2016).Nonamplification Sandwich Assay Platform for Sensitive Nucleic Acid Detection Based on AuNPs Enumeration with the Dark-Field Microscope.ANALYTICAL CHEMISTRY.
MLA	Li, Tian,et al."Nonamplification Sandwich Assay Platform for Sensitive Nucleic Acid Detection Based on AuNPs Enumeration with the Dark-Field Microscope".ANALYTICAL CHEMISTRY (2016).