Near-Infrared Fluorogenic Probes with Polarity-Sensitive Emission for in   Vivo Imaging of an Ovarian Cancer Biomarker

doi:10.1021/acsami.5b11826

CORC > 北京大学 > 化学与分子工程学院

	Near-Infrared Fluorogenic Probes with Polarity-Sensitive Emission for in Vivo Imaging of an Ovarian Cancer Biomarker
	Yao, Defan ; Lin, Zhi ; Wu, Junchen
刊名	ACS APPLIED MATERIALS & INTERFACES
	2016
关键词	lysophosphatidic acid biomarker polarity-sensitive near-infrared probe AGGREGATION-INDUCED EMISSION LIGHT-UP BIOPROBE LYSOPHOSPHATIDIC ACID FLUORESCENT-PROBE SQUARAINE DYE CELLS RECEPTOR INHIBITOR AUTOTAXIN NANOPARTICLES
DOI	10.1021/acsami.5b11826
英文摘要	Lysophosphatidic acid (LPA, cutoff values >= 1.5 mu M) is an effective biomarker for early stage ovarian cancer. The development of selective probes for LPA detection is therefore critical for early clinical diagnosis. Although current methods have been developed for the detection of LPA in solution, they cannot be used for tracking LPA in vivo. Here, we report a near-infrared (NIR) fluorescent probe that can selectively respond to LPA based on polarity-sensitive emission at a very low detection limit of 0.5 mu M in situ. This probe exhibits a marked increase of fluorescence at 720 nm upon binding to LPA, allowing the direct visualization of LPA in vitro and in vivo without interference from other biomolecules. Moreover, the probe containing two arginine-glycine-aspartic acid units can be efficiently taken up by cancer cells based on an alpha(v)beta(3) integrin receptor targeting mechanism. It also exhibits excellent biocompatibility and high pH stability in live cells and in vivo. Confocal laser scanning microscopy and flow cytometric imaging of SKOV-3 cells have confirmed that our probe can be used to image LPA in live cells. In particular, its NIR turn-on fluorescence can be used to effectively monitor LPA imaging in a SKOV-3 tumor-bearing mouse model. Our probe may pave the way for the detection of cancer-related biomarkers and even for early stage cancer diagnosis.; National Basic Research 973 Program [2013CB733700]; NSFC/China [91529101, 21572057]; Program of Introducing Talents of Discipline to Universities [B16017]; Fundamental Research Funds for the Central Universities [WJ1213007]; SCI(E); EI; PubMed; ARTICLE; jcwu@ecust.edu.cn; 9; 5847-5856; 8
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/434768]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Yao, Defan,Lin, Zhi,Wu, Junchen. Near-Infrared Fluorogenic Probes with Polarity-Sensitive Emission for in Vivo Imaging of an Ovarian Cancer Biomarker[J]. ACS APPLIED MATERIALS & INTERFACES,2016.
APA	Yao, Defan,Lin, Zhi,&Wu, Junchen.(2016).Near-Infrared Fluorogenic Probes with Polarity-Sensitive Emission for in Vivo Imaging of an Ovarian Cancer Biomarker.ACS APPLIED MATERIALS & INTERFACES.
MLA	Yao, Defan,et al."Near-Infrared Fluorogenic Probes with Polarity-Sensitive Emission for in Vivo Imaging of an Ovarian Cancer Biomarker".ACS APPLIED MATERIALS & INTERFACES (2016).