In vivo metabolic labeling of sialoglycans in the mouse brain by using a   liposome-assisted bioorthogonal reporter strategy

doi:10.1073/pnas.1516524113

CORC > 北京大学 > 化学与分子工程学院

	In vivo metabolic labeling of sialoglycans in the mouse brain by using a liposome-assisted bioorthogonal reporter strategy
	Xie, Ran ; Dong, Lu ; Du, Yifei ; Zhu, Yuntao ; Hua, Rui ; Zhang, Chen ; Chen, Xing
刊名	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
	2016
关键词	brain sialic acid live imaging glycoproteomics histochemistry SIALIC-ACID POLYSIALIC ACID LIVING ANIMALS NERVOUS-SYSTEM SYNAPTIC-TRANSMISSION ESSENTIAL NUTRIENT MODIFIED PROTEINS RAT-BRAIN GANGLIOSIDES BARRIER
DOI	10.1073/pnas.1516524113
英文摘要	Mammalian brains are highly enriched with sialoglycans, which have been implicated in brain development and disease progression. However, in vivo labeling and visualization of sialoglycans in the mouse brain remain a challenge because of the blood-brain barrier. Here we introduce a liposome-assisted bioorthogonal reporter (LABOR) strategy for shuttling 9-azido sialic acid (9AzSia), a sialic acid reporter, into the brain to metabolically label sialoglycoconjugates, including sialylated glycoproteins and glycolipids. Subsequent bioorthogonal conjugation of the incorporated 9AzSia with fluorescent probes via click chemistry enabled fluorescence imaging of brain sialoglycans in living animals and in brain sections. Newly synthesized sialoglycans were found to widely distribute on neuronal cell surfaces, in particular at synaptic sites. Furthermore, large-scale proteomic profiling identified 140 brain sialylated glycoproteins, including a wealth of synapse-associated proteins. Finally, by performing a pulse-chase experiment, we showed that dynamic sialylation is spatially regulated, and that turnover of sialoglycans in the hippocampus is significantly slower than that in other brain regions. The LABOR strategy provides a means to directly visualize and monitor the sialoglycan biosynthesis in the mouse brain and will facilitate elucidating the functional role of brain sialylation.; National Natural Science Foundation of China [21425204, 91313301]; National Basic Research Program of China [2012CB917303]; SCI(E); PubMed; ARTICLE; xingchen@pku.edu.cn; 19; 5173-5178; 113
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/433923]
专题	化学与分子工程学院生命科学学院心理与认知科学学院
推荐引用方式 GB/T 7714	Xie, Ran,Dong, Lu,Du, Yifei,et al. In vivo metabolic labeling of sialoglycans in the mouse brain by using a liposome-assisted bioorthogonal reporter strategy[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2016.
APA	Xie, Ran.,Dong, Lu.,Du, Yifei.,Zhu, Yuntao.,Hua, Rui.,...&Chen, Xing.(2016).In vivo metabolic labeling of sialoglycans in the mouse brain by using a liposome-assisted bioorthogonal reporter strategy.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA.
MLA	Xie, Ran,et al."In vivo metabolic labeling of sialoglycans in the mouse brain by using a liposome-assisted bioorthogonal reporter strategy".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2016).