Nanoprobes for two-photon excitation time-resolved imaging of living   animals: In situ analysis of tumor-targeting dynamics of nanocarriers

doi:10.1016/j.biomaterials.2016.05.032

CORC > 北京大学 > 化学与分子工程学院

	Nanoprobes for two-photon excitation time-resolved imaging of living animals: In situ analysis of tumor-targeting dynamics of nanocarriers
	Yang, Wen ; Fu, Li-Min ; Wen, Xue ; Liu, Ying ; Tian, Ye ; Liu, Yu-Chen ; Han, Rong-Cheng ; Gao, Zhi-Yue ; Wang, Tian-En ; Sha, Yin-Lin ; Jiang, Yu-Qiang ; Wang, Yuan ; Zhang, Jian-Ping
刊名	BIOMATERIALS
	2016
关键词	Nanoprobes Nanocarriers Tumor-targeting dynamics Two-photon-excitation Time-resolved imaging Noninvasive luminescence analysis CANCER-THERAPY EUROPIUM COMPLEX DRUG-DELIVERY QUANTUM DOTS NANOPARTICLES LUMINESCENCE TRANSFERRIN MICROSCOPY NM ACCUMULATION
DOI	10.1016/j.biomaterials.2016.05.032
英文摘要	Great challenges remain in the noninvasive luminescence imaging analysis of tumor-targeting dynamics of nanocarriers in living animals which is of significance for the development of anti-cancer nano medicine. In this work, luminescent nanoparticles Eu(tta)(3)bpt@SMA (d(av), = 15 nm), which exhibited good water dispersion stability and high yields of red Eu-luminescence under near-infrared two-photon excitation, were prepared by a modified microfluidic mixing method in the absence of surfactants. Tumor-targeting agents, Arg-Gly-Asp-D-Phe-Lys (cRGD) polypeptide or transferrin (Tf), were then anchored on the nanoparticle surfaces to form the desired nanocarriers Eu@SMA-RGD or Eu@SMA-Tf. The tumor-targeting processes of the prepared nanocarriers in intact living mice were analyzed on a home-built two-photon excitation time-resolved (TPE-TR) imaging apparatus having a wide view filed. The TPE-TR strategy could effectively suppress the interference from biological autofluorescence, which allowed the targeted domains to be visualized with a high signal-to-noise ratio. It was found that the tumor-tissue trapping efficacy of Eu@SMA-RGD was much higher than that of Eu@SMA-Tf, and the desorption process from the tumor tissues of Eu@SMA-RGD was slower than that of Eu@SMA-Tf. The methods developed in this work pave a way to investigate the in vivo tumor-targeting dynamics of nanocarriers by noninvasive luminescence imaging of living animals. (C) 2016 Elsevier Ltd. All rights reserved.; NSFC [21227803, 21133001, 21373268, 21573010]; SCI(E); EI; PubMed; ARTICLE; wangy@pku.edu.cn; jpzhang@chem.ruc.edu.cn; 152-161; 100
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/433480]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Yang, Wen,Fu, Li-Min,Wen, Xue,et al. Nanoprobes for two-photon excitation time-resolved imaging of living animals: In situ analysis of tumor-targeting dynamics of nanocarriers[J]. BIOMATERIALS,2016.
APA	Yang, Wen.,Fu, Li-Min.,Wen, Xue.,Liu, Ying.,Tian, Ye.,...&Zhang, Jian-Ping.(2016).Nanoprobes for two-photon excitation time-resolved imaging of living animals: In situ analysis of tumor-targeting dynamics of nanocarriers.BIOMATERIALS.
MLA	Yang, Wen,et al."Nanoprobes for two-photon excitation time-resolved imaging of living animals: In situ analysis of tumor-targeting dynamics of nanocarriers".BIOMATERIALS (2016).