Bioorthogonal Chemical Activation of Kinases in Living Systems

doi:10.1021/acscentsci.6b00024

CORC > 北京大学 > 化学与分子工程学院

	Bioorthogonal Chemical Activation of Kinases in Living Systems
	Zhang, Gong ; Li, Jie ; Xie, Ran ; Fan, Xinyuan ; Liu, Yanjun ; Zheng, Siqi ; Ge, Yun ; Chen, Peng R. g
刊名	ACS CENTRAL SCIENCE
	2016
关键词	TETRAZINE LIGATION PROTEIN FUNCTION GENETIC-CODE CELLS STRATEGIES CHEMISTRY FAMILY SRC
DOI	10.1021/acscentsci.6b00024
英文摘要	Selective manipulation of protein kinases under living conditions is highly desirable yet extremely challenging, particularly in a gain-of-function fashion. Here we employ our recently developed bioorthogonal cleavage reaction as a general strategy for intracellular activation of individual kinases. Site-specific incorporation of trans-cyclooctene-caged lysine in place of the conserved catalytic lysine, in conjunction with the cleavage partner dimethyl-tetrazine, allowed efficient lysine decaging with the kinase activity chemically rescued in living systems.; SCI(E); PubMed; ARTICLE; pengchen@pku.edu.cn; 5; 325-331; 2
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/433409]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Zhang, Gong,Li, Jie,Xie, Ran,et al. Bioorthogonal Chemical Activation of Kinases in Living Systems[J]. ACS CENTRAL SCIENCE,2016.
APA	Zhang, Gong.,Li, Jie.,Xie, Ran.,Fan, Xinyuan.,Liu, Yanjun.,...&Chen, Peng R. g.(2016).Bioorthogonal Chemical Activation of Kinases in Living Systems.ACS CENTRAL SCIENCE.
MLA	Zhang, Gong,et al."Bioorthogonal Chemical Activation of Kinases in Living Systems".ACS CENTRAL SCIENCE (2016).