Design, preparation, and selection of DNA-encoded dynamic libraries

doi:10.1039/c5sc02467f

CORC > 北京大学 > 化学与分子工程学院

	Design, preparation, and selection of DNA-encoded dynamic libraries
	Li, Gang ; Zheng, Wenlu ; Chen, Zitian ; Zhou, Yu ; Liu, Yu ; Yang, Junrui ; Huang, Yanyi ; Li, Xiaoyu
刊名	CHEMICAL SCIENCE
	2015
关键词	SMALL-MOLECULE LIBRARIES TEMPLATE-DIRECTED SYNTHESIS PROTEIN-BINDING AGENTS LIGAND-TARGET PAIRS PHOTO-CROSS-LINKING IN-VITRO SELECTION COMBINATORIAL CHEMISTRY CHEMICAL LIBRARIES ADAPTIVE CHEMISTRY COVALENT CHEMISTRY
DOI	10.1039/c5sc02467f
英文摘要	We report a method for the preparation and selection of DNA-encoded dynamic libraries (DEDLs). The library is composed of two sets of DNA-linked small molecules that are under dynamic exchange through DNA hybridization. Addition of the protein target shifted the equilibrium, favouring the assembly of high affinity bivalent binders. Notably, we introduced a novel locking mechanism to stop the dynamic exchange and "freeze" the equilibrium, thereby enabling downstream hit isolation and decoding by PCR amplification and DNA sequencing. Our DEDL approach has circumvented the limitation of library size and realized the analysis and selection of large dynamic libraries. In addition, this method also eliminates the requirement for modified and immobilized target proteins.; Ministry of Science and Technology Basic Research Program [2011CB809100]; NSFC [21272016, 21002003, 91013003]; Doctoral Fund of Ministry of Education of China [20120001110083]; SCI(E); EI; ARTICLE; xiaoyuli@pku.edu.cn; 12; 7097-7104; 6
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/424618]
专题	化学与分子工程学院工学院
推荐引用方式 GB/T 7714	Li, Gang,Zheng, Wenlu,Chen, Zitian,et al. Design, preparation, and selection of DNA-encoded dynamic libraries[J]. CHEMICAL SCIENCE,2015.
APA	Li, Gang.,Zheng, Wenlu.,Chen, Zitian.,Zhou, Yu.,Liu, Yu.,...&Li, Xiaoyu.(2015).Design, preparation, and selection of DNA-encoded dynamic libraries.CHEMICAL SCIENCE.
MLA	Li, Gang,et al."Design, preparation, and selection of DNA-encoded dynamic libraries".CHEMICAL SCIENCE (2015).