Bisulfite-free, base-resolution analysis of 5-formylcytosine at the   genome scale

doi:10.1038/NMETH.3569

CORC > 北京大学 > 化学与分子工程学院

	Bisulfite-free, base-resolution analysis of 5-formylcytosine at the genome scale
	Xia, Bo ; Han, Dali ; Lu, Xingyu ; Sun, Zhaozhu ; Zhou, Ankun ; Yin, Qiangzong ; Zeng, Hu ; Liu, Menghao ; Jiang, Xiang ; Xie, Wei ; He, Chuan ; Yi, Chengqi
刊名	NATURE METHODS
	2015
关键词	THYMINE DNA GLYCOSYLASE MAMMALIAN DNA 5-HYDROXYMETHYLCYTOSINE 5-CARBOXYLCYTOSINE 5-METHYLCYTOSINE DYNAMICS REVEALS TET TRANSCRIPTION PROTEINS
DOI	10.1038/NMETH.3569
英文摘要	Active DNA demethylation in mammals involves oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC). However, genome-wide detection of 5fC at single-base resolution remains challenging. Here we present fC-CET, a bisulfite-free method for whole-genome analysis of 5fC based on selective chemical labeling of 5fC and subsequent C-to-T transition during PCR. Base-resolution 5fC maps showed limited overlap with 5hmC, with 5fC-marked regions more active than 5hmC-marked ones.; National Basic Research Foundation of China [2014CB964900]; National Natural Science Foundation of China [31270838, 21472009]; US National Institutes of Health [R01 HG006827]; Howard Hughes Medical Institute; SCI(E); PubMed; ARTICLE; chuanhe@uchicago.edu; chengqi.yi@pku.edu.cn; 11; 1047-1050; 12
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/418172]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Xia, Bo,Han, Dali,Lu, Xingyu,et al. Bisulfite-free, base-resolution analysis of 5-formylcytosine at the genome scale[J]. NATURE METHODS,2015.
APA	Xia, Bo.,Han, Dali.,Lu, Xingyu.,Sun, Zhaozhu.,Zhou, Ankun.,...&Yi, Chengqi.(2015).Bisulfite-free, base-resolution analysis of 5-formylcytosine at the genome scale.NATURE METHODS.
MLA	Xia, Bo,et al."Bisulfite-free, base-resolution analysis of 5-formylcytosine at the genome scale".NATURE METHODS (2015).