毛细管电泳-激光诱导荧光检测分析重组人促红细胞生成素时不同荧光衍生化方法的比较研究(英文)

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	毛细管电泳-激光诱导荧光检测分析重组人促红细胞生成素时不同荧光衍生化方法的比较研究(英文); Comparative study on different derivatization procedures for analysis of recombinant human erythropoietin by capillary electrophoresis with laser-induced fluorescence detection
	杨霞 ; 庞楠楠 ; 付晓芳 ; 尹红锋 ; 廖一平 ; 刘虎威
刊名	Journal of Chinese Pharmaceutical Sciences
	2014
关键词	重组人促红细胞生成素毛细管电泳激光诱导荧光检测在线荧光标记 Recombinant human erythropoietin Capillary electrophoresis Laser-induced fluorescence detection On-capillary derivatization
英文摘要	内源性糖蛋白人促红细胞生成素(hEPO)在控制红细胞生成方面具有重要的作用。而重组EPO(rhEPO)则是一种基因重组药物,属于高度糖基化的糖蛋白,在临床上多用于促进术后康复和体能恢复。同时,作为一种内源性激素,它对红细胞生成有特异性的刺激作用,对机体的有氧工作能力有明显的促进作用,故在一些耐力运动比赛项目中常被滥用。由于其明显的副作用,国际奥委会已将rhEPO列为违禁药物。又由于rhEPO与人体自然生成的hEPO在序列结构上几乎没有区别,而且rhEPO的半衰期很短,在血液和尿液中的浓度非常低,所以药检难度很大。目前,国际反兴奋剂委员会的rhEPO检测方法是基于尿检和血检的综合结果。此方法虽然...; Human erythropoietin (hEPO), an endogenous glycoprotein, plays a fundamental role in erythropoiesis controlling the formation of red blood cells. Production of recombinant human erythropoietin (rhEPO) has made it possible for its abuse in competitive sports. In this work, pre-capillary and on-capillary derivatization by 5-furoylquinoline-3-carboxaldehyde (FQ) and fluorescein isothiocyanate (FITC) for the detection of rhEPO by capillary electrophoresis with laserinduced fluorescence detection (CE-LIF) were compared. FQ pre-capillary labeling improves sensitivity but degrades the glycoforms separation due to the inhomogeneity of the reaction products from multiple labeling. Compared with FITC pre-capillary derivatization with the excess fluorescent background, the on-capillary FQ derivatization method can provide shorter analysis time, lower background, and better selectivity. It is demonstrated that, through optimizing reaction conditions of FQ on-capillary derivatization, both high sensitivity and satisfactory resolution for the analysis of the glycoforms of rhEPO could be obtained. This method can be used for the glycoforms profiling and quality control of rhEPO. It may be used as a candidate method for fast screening in doping control analysis.; 中国科学引文数据库(CSCD); 05; 317-323; 23
语种	中文
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/416221]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	杨霞,庞楠楠,付晓芳,等. 毛细管电泳-激光诱导荧光检测分析重组人促红细胞生成素时不同荧光衍生化方法的比较研究(英文), Comparative study on different derivatization procedures for analysis of recombinant human erythropoietin by capillary electrophoresis with laser-induced fluorescence detection[J]. Journal of Chinese Pharmaceutical Sciences,2014.
APA	杨霞,庞楠楠,付晓芳,尹红锋,廖一平,&刘虎威.(2014).毛细管电泳-激光诱导荧光检测分析重组人促红细胞生成素时不同荧光衍生化方法的比较研究(英文).Journal of Chinese Pharmaceutical Sciences.
MLA	杨霞,et al."毛细管电泳-激光诱导荧光检测分析重组人促红细胞生成素时不同荧光衍生化方法的比较研究(英文)".Journal of Chinese Pharmaceutical Sciences (2014).