Comprehensive Analysis of NAC Domain Transcription Factor Gene Family in Populus trichocarpa

CORC > 青岛生物能源与过程研究所 > 中国科学院青岛生物能源与过程研究所 > 植物代谢工程团队

	Comprehensive Analysis of NAC Domain Transcription Factor Gene Family in Populus trichocarpa
	Hu, Ruibo; Qi, Guang; Kong, Yingzhen; Kong, Dejing; Gao, Qian; Zhou, Gongke
刊名	BMC PLANT BIOLOGY
	2010-07-15
卷号	10 期号:1
ISSN号	1471-2229
中文摘要	Background: NAC (NAM, ATAF1/2 and CUC2) domain proteins are plant-specific transcriptional factors known to play diverse roles in various plant developmental processes. NAC transcription factors comprise of a large gene family represented by more than 100 members in Arabidopsis, rice and soybean etc. Recently, a preliminary phylogenetic analysis was reported for NAC gene family from 11 plant species. However, no comprehensive study incorporating phylogeny, chromosomal location, gene structure, conserved motifs, and expression profiling analysis has been presented thus far for the model tree species Populus. Results: In the present study, a comprehensive analysis of NAC gene family in Populus was performed. A total of 163 full-length NAC genes were identified in Populus, and they were phylogeneticly clustered into 18 distinct subfamilies. The gene structure and motif compositions were considerably conserved among the subfamilies. The distributions of 120 Populus NAC genes were non-random across the 19 linkage groups (LGs), and 87 genes (73%) were preferentially retained duplicates that located in both duplicated regions. The majority of NACs showed specific temporal and spatial expression patterns based on EST frequency and microarray data analyses. However, the expression patterns of a majority of duplicate genes were partially redundant, suggesting the occurrence of subfunctionalization during subsequent evolutionary process. Furthermore, quantitative real-time RT-PCR (RT-qPCR) was performed to confirm the tissue-specific expression patterns of 25 NAC genes. Conclusion: Based on the genomic organizations, we can conclude that segmental duplications contribute significantly to the expansion of Populus NAC gene family. The comprehensive expression profiles analysis provides first insights into the functional divergence among members in NAC gene family. In addition, the high divergence rate of expression patterns after segmental duplications indicates that NAC genes in Populus are likewise to have been retained by substantial subfunctionalization. Taken together, our results presented here would be helpful in laying the foundation for functional characterization of NAC gene family and further gaining an understanding of the structure-function relationship between these family members.
英文摘要	Background: NAC (NAM, ATAF1/2 and CUC2) domain proteins are plant-specific transcriptional factors known to play diverse roles in various plant developmental processes. NAC transcription factors comprise of a large gene family represented by more than 100 members in Arabidopsis, rice and soybean etc. Recently, a preliminary phylogenetic analysis was reported for NAC gene family from 11 plant species. However, no comprehensive study incorporating phylogeny, chromosomal location, gene structure, conserved motifs, and expression profiling analysis has been presented thus far for the model tree species Populus.
学科主题	能源植物资源
WOS标题词	Science & Technology ; Life Sciences & Biomedicine
类目[WOS]	Plant Sciences
研究领域[WOS]	Plant Sciences
关键词[WOS]	GENOME-WIDE ANALYSIS ; SECONDARY WALL SYNTHESIS ; LATERAL ROOT DEVELOPMENT ; STRESS-RESPONSIVE GENES ; NO-APICAL-MERISTEM ; ARABIDOPSIS-THALIANA ; MOLECULAR CHARACTERIZATION ; FUNCTIONAL-ANALYSIS ; CAMBIAL MERISTEM ; DUPLICATED GENES
收录类别	SCI
语种	英语
WOS记录号	WOS:000280841400001
公开日期	2011-09-09
内容类型	期刊论文
源URL	[http://ir.qibebt.ac.cn//handle/337004/632]
专题	青岛生物能源与过程研究所_植物代谢工程团队
作者单位	Chinese Acad Sci, Qingdao Inst BioEnergy & Bioproc Technol, Qingdao 266101, Peoples R China
推荐引用方式 GB/T 7714	Hu, Ruibo,Qi, Guang,Kong, Yingzhen,et al. Comprehensive Analysis of NAC Domain Transcription Factor Gene Family in Populus trichocarpa[J]. BMC PLANT BIOLOGY,2010,10(1).
APA	Hu, Ruibo,Qi, Guang,Kong, Yingzhen,Kong, Dejing,Gao, Qian,&Zhou, Gongke.(2010).Comprehensive Analysis of NAC Domain Transcription Factor Gene Family in Populus trichocarpa.BMC PLANT BIOLOGY,10(1).
MLA	Hu, Ruibo,et al."Comprehensive Analysis of NAC Domain Transcription Factor Gene Family in Populus trichocarpa".BMC PLANT BIOLOGY 10.1(2010).