Production and characterization of monoclonal antibody against recombinant human erythropoietin

CORC > 北京大学 > 化学与分子工程学院

	Production and characterization of monoclonal antibody against recombinant human erythropoietin
	Mi Jie-Bo ; Yan Jin ; Ding Xiao-Jie ; Guo Zhen-Quan ; Zhao Mei-Ping ; Chang Wen-Bao
刊名	biomedical and environmental sciences bes
	2007
英文摘要	To produce specific monoclonal antibody (mAb) against recombinant human erythropoietin (rHuEPO) for development of highly efficient methods for erythropoietin detection in biological fluids.rHuEPO was covalently coupled with bovine serum albumin (BSA) and the conjugate was used to immunize mice to produce specific mAb against rHuEPO based on hybridoma technology. The obtained F3-mAb was characterized by enzyme-linked immunosorbent assay (ELISA), SDS-PAGE and Western blot.The isotype of F3-mAb was found to be IgM with an affinity constant of 2.1 x 10(8) L/mol. The competitive ELISA using the obtained IgM showed a broader linear range and lower detection limit compared with previous work.The modification of rHuEPO was proved to be successful in generating required specific mAb with high avidity to rHuEPO.; SCI(E); PubMed; 中国科学引文数据库(CSCD); 0; ARTICLE; 3; 184-8; 20
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/345370]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Mi Jie-Bo,Yan Jin,Ding Xiao-Jie,et al. Production and characterization of monoclonal antibody against recombinant human erythropoietin[J]. biomedical and environmental sciences bes,2007.
APA	Mi Jie-Bo,Yan Jin,Ding Xiao-Jie,Guo Zhen-Quan,Zhao Mei-Ping,&Chang Wen-Bao.(2007).Production and characterization of monoclonal antibody against recombinant human erythropoietin.biomedical and environmental sciences bes.
MLA	Mi Jie-Bo,et al."Production and characterization of monoclonal antibody against recombinant human erythropoietin".biomedical and environmental sciences bes (2007).