Lipid profiling of rat peritoneal surface layers by online normal- and   reversed-phase 2D LC QToF-MS

doi:10.1194/jlr.D007567

CORC > 北京大学 > 化学与分子工程学院

	Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS
	Nie, Honggang ; Liu, Ranran ; Yang, Youyou ; Bai, Yu ; Guan, Yafeng ; Qian, Daqing ; Wang, Tao ; Liu, Huwei
刊名	类脂研究杂志
	2010
关键词	lipidomic free fatty acid phospholipid lyso-phospholipid plasmalogen peritoneal dialysis peritoneum biomarker two-dimensional liquid chromatography mass spectrometry PERFORMANCE LIQUID-CHROMATOGRAPHY IONIZATION MASS-SPECTROMETRY LIGHT-SCATTERING DETECTION CAPILLARY GAS-CHROMATOGRAPHY FREE FATTY-ACIDS ELECTROSPRAY-IONIZATION FRAGMENTATION PROCESSES RAPID SEPARATION NEUTRAL LIPIDS BIOLOGICAL SAMPLES
DOI	10.1194/jlr.D007567
英文摘要	An online, two-dimensional (2D) liquid chromatography (LC) quadrupole time-of-flight mass spectrometry (QToF-MS) method was developed for lipid profiling of rat peritoneal surface layers, in which the lipid classes and species could be simultaneously separated in one injection with a significantly increased sensitivity. Different lipid classes were separated on a normal-phase column in the first dimension and lipid molecular species were separated on a reversed-phase column in the second dimension, so that the ion suppression effects were reduced while the detection sensitivity was improved. Identified were 721 endogenous lipid species from 12 lipid classes, in which 415 structures were confirmed using tandem mass spectra, and the other 306 lipid molecular species were identified by accurate masses. The linearity, limit of detection, and repeatability were all satisfactory. The method was applied to the investigation of the lipid changes in rat peritoneal surface layer after peritoneal dialysis, and 32 potential lipid biomarkers were identified, as their concentrations in the dosed group were 2.2-12.5 times of those in the control group. The results revealed that this 2D LC-MS system was a promising tool for lipid profiling of complex biological samples.-Nie, H., R. Liu, Y. Yang, Y. Bai, Y. Guan, D. Qian, T. Wang, and H. Liu. Lipid profiling of rat peritoneal surface layers by online normal-and reversed-phase 2D LC QToF-MS. J. Lipid Res. 2010. 51: 2833-2844.; Biochemistry & Molecular Biology; SCI(E); PubMed; 34; ARTICLE; 9; 2833-2844; 51
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/344227]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Nie, Honggang,Liu, Ranran,Yang, Youyou,et al. Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS[J]. 类脂研究杂志,2010.
APA	Nie, Honggang.,Liu, Ranran.,Yang, Youyou.,Bai, Yu.,Guan, Yafeng.,...&Liu, Huwei.(2010).Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS.类脂研究杂志.
MLA	Nie, Honggang,et al."Lipid profiling of rat peritoneal surface layers by online normal- and reversed-phase 2D LC QToF-MS".类脂研究杂志 (2010).