D-myo-Inositol-3-Phosphate Affects Phosphatidylinositol-Mediated   Endomembrane Function in Arabidopsis and Is Essential for   Auxin-Regulated Embryogenesis

doi:10.1105/tpc.111.083337

CORC > 北京大学 > 化学与分子工程学院

	D-myo-Inositol-3-Phosphate Affects Phosphatidylinositol-Mediated Endomembrane Function in Arabidopsis and Is Essential for Auxin-Regulated Embryogenesis
	Luo, Yu ; Qin, Genji ; Zhang, Jun ; Liang, Yuan ; Song, Yingqi ; Zhao, Meiping ; Tsuge, Tomohiko ; Aoyama, Takashi ; Liu, Jingjing ; Gu, Hongya ; Qu, Li-Jia
刊名	plant cell
	2011
关键词	MYO-INOSITOL-1-PHOSPHATE SYNTHASE MYO-INOSITOL CELL-DEATH SACCHAROMYCES-CEREVISIAE MYOINOSITOL METABOLISM PLANT DEVELOPMENT SEED DEVELOPMENT GENE BIOSYNTHESIS THALIANA
DOI	10.1105/tpc.111.083337
英文摘要	In animal cells, myo-inositol is an important regulatory molecule in several physiological and biochemical processes, including signal transduction and membrane biogenesis. However, the fundamental biological functions of myo-inositol are still far from clear in plants. Here, we report the genetic characterization of three Arabidopsis thaliana genes encoding D-myo-inositol-3-phosphate synthase (MIPS), which catalyzes the rate-limiting step in de novo synthesis of myo-inositol. Each of the three MIPS genes rescued the yeast ino1 mutant, which is defective in yeast MIPS gene INO1, and they had different dynamic expression patterns during Arabidopsis embryo development. Although single mips mutants showed no obvious phenotypes, the mips1 mips2 double mutant and the mips1 mips2 mips3 triple mutant were embryo lethal, whereas the mips1 mips3 and mips1 mips2(+/-) double mutants had abnormal embryos. The mips phenotypes resembled those of auxin mutants. Indeed, the double and triple mips mutants displayed abnormal expression patterns of DR5:green fluorescent protein, an auxin-responsive fusion protein, and they had altered PIN1 subcellular localization. Also, membrane trafficking was affected in mips1 mips3. Interestingly, overexpression of PHOSPHATIDYLINOSITOL SYNTHASE2, which converts myoinositol to membrane phosphatidylinositol (PtdIns), largely rescued the cotyledon and endomembrane defects in mips1 mips3. We conclude that myo-inositol serves as the main substrate for synthesizing PtdIns and phosphatidylinositides, which are essential for endomembrane structure and trafficking and thus for auxin-regulated embryogenesis.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000291000500017&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Plant Sciences; Cell Biology; SCI(E); PubMed; 34; ARTICLE; 4; 1352-1372; 23
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/319964]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Luo, Yu,Qin, Genji,Zhang, Jun,et al. D-myo-Inositol-3-Phosphate Affects Phosphatidylinositol-Mediated Endomembrane Function in Arabidopsis and Is Essential for Auxin-Regulated Embryogenesis[J]. plant cell,2011.
APA	Luo, Yu.,Qin, Genji.,Zhang, Jun.,Liang, Yuan.,Song, Yingqi.,...&Qu, Li-Jia.(2011).D-myo-Inositol-3-Phosphate Affects Phosphatidylinositol-Mediated Endomembrane Function in Arabidopsis and Is Essential for Auxin-Regulated Embryogenesis.plant cell.
MLA	Luo, Yu,et al."D-myo-Inositol-3-Phosphate Affects Phosphatidylinositol-Mediated Endomembrane Function in Arabidopsis and Is Essential for Auxin-Regulated Embryogenesis".plant cell (2011).