Studies on the thermokinetic characterisation of actin polymerization   and the effect of cisplatin

doi:10.1016/0141-8130(95)01067-X

CORC > 北京大学 > 化学与分子工程学院

	Studies on the thermokinetic characterisation of actin polymerization and the effect of cisplatin
	Zeng, HH ; Wang, K ; Wang, BH ; Zhang, YM
刊名	国际生物大分子杂志
	1996
关键词	actin polymerization cisplatin thermokinetics
DOI	10.1016/0141-8130(95)01067-X
英文摘要	The microcalorimetic measurement for studying the thermokinetic of actin polymeriztion in vitro was employed for the first time. The thermodynamic parameters of actin polymerization at 310.15 K are reported as Delta H-m = 49.26 kJ/mol, Delta G(m) = 25.62 kJ/mol and Delta S-m = 241.54 J/K.mol. The thermogram shows that polymerization of actin is a complicate process in which multistep reactions occur corresponding to exothermic and endothermic effects. Cisplatin, at lower concentration, affects the polymerization of G-actin resulting in a decrease of Delta H-m; and, at higher concentration, induce the crosslinking and depolymerization of F-actin in the equilibrium system of G/F. The experimental results show that as the concentration of cisplatin increases, the sign of Delta H-m changes from positive to negative, i.e. the magnitude and sign of Delta H-m of actin polymerization depends on the concentration of cisplatin.; Biochemistry & Molecular Biology; SCI(E); 11; ARTICLE; 3; 161-166; 18
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/258422]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Zeng, HH,Wang, K,Wang, BH,et al. Studies on the thermokinetic characterisation of actin polymerization and the effect of cisplatin[J]. 国际生物大分子杂志,1996.
APA	Zeng, HH,Wang, K,Wang, BH,&Zhang, YM.(1996).Studies on the thermokinetic characterisation of actin polymerization and the effect of cisplatin.国际生物大分子杂志.
MLA	Zeng, HH,et al."Studies on the thermokinetic characterisation of actin polymerization and the effect of cisplatin".国际生物大分子杂志 (1996).