A protein interaction network for the analysis of the neuronal   differentiation of neural stem cells in response to titanium dioxide   nanoparticles

doi:10.1016/j.biomaterials.2009.12.054

CORC > 北京大学 > 化学与分子工程学院

	A protein interaction network for the analysis of the neuronal differentiation of neural stem cells in response to titanium dioxide nanoparticles
	Liu, Xiaoyan ; Ren, Xiufang ; Deng, Xiaoyong ; Huo, Yinan ; Xie, Jiang ; Huang, Hai ; Jiao, Zheng ; Wu, Minghong ; Liu, Yuanfang ; Wen, Tieqiao
刊名	biomaterials
	2010
关键词	Titanium dioxide nanoparticles Neural stem cells Differentiation Protein-protein interaction Proteomics HUMAN DERMAL FIBROBLASTS EPITHELIAL-CELLS PROGENITOR CELLS IN-VITRO GENE-EXPRESSION RAT ACTIVATION RECEPTORS EPSILON NEUROPROTECTION
DOI	10.1016/j.biomaterials.2009.12.054
英文摘要	The effects of titanium dioxide (TiO(2)) nanoparticles (NPs) on the differentiation of neural stem cells are reported. Our findings indicate that TiO(2) NPs lead to a differentiational tendency towards neurons from neural stem cells, suggesting TiO(2) NPs might be a beneficial inducer for neuronal differentiation. To insight into the possible molecular mechanism of the neuronal differentiation, we conducted a protein-protein interaction network (PIN) analysis. To this end, a global mapping of target proteins induced by TiO(2) NPs was first made by a 2-dimensional electrophoresis analysis. Results showed that 9 proteins were significantly changed and then they were subjected to the mass spectrometric assay. All 9 identified proteins are involved in signal, molecular chaperones, cytoskeleton, and nucleoprotein. Further, based on our experimental data and DIP, IntAct-EBI, GRID database, a protein-protein interaction network was constructed, which provides highly integrated information exhibiting the protein-protein interaction. By analysis of the gene expression, the signal pathway involving Cx43 phosphotylation, which is negatively regulated by the protein kinase C epsilon (PKC epsilon), is demonstrated. It is inferred that PKCE plays a pivotal negative role in the neuronal differentiation of stem neural cells in response to the TiO(2) NPs exposure. (C) 2009 Elsevier Ltd. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000275946500013&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Engineering, Biomedical; Materials Science, Biomaterials; SCI(E); EI; 21; ARTICLE; 11; 3063-3070; 31
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/243974]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Liu, Xiaoyan,Ren, Xiufang,Deng, Xiaoyong,et al. A protein interaction network for the analysis of the neuronal differentiation of neural stem cells in response to titanium dioxide nanoparticles[J]. biomaterials,2010.
APA	Liu, Xiaoyan.,Ren, Xiufang.,Deng, Xiaoyong.,Huo, Yinan.,Xie, Jiang.,...&Wen, Tieqiao.(2010).A protein interaction network for the analysis of the neuronal differentiation of neural stem cells in response to titanium dioxide nanoparticles.biomaterials.
MLA	Liu, Xiaoyan,et al."A protein interaction network for the analysis of the neuronal differentiation of neural stem cells in response to titanium dioxide nanoparticles".biomaterials (2010).