High-throughput intracellular pteridinic profiling by liquid   chromatography-quadrupole time-of-flight mass spectrometry

doi:10.1016/j.aca.2014.10.044

CORC > 北京大学 > 化学与分子工程学院

	High-throughput intracellular pteridinic profiling by liquid chromatography-quadrupole time-of-flight mass spectrometry
	Burton, Casey ; Weng, Rui ; Yang, Li ; Bai, Yu ; Liu, Huwei ; Ma, Yinfa
刊名	analytica chimica acta
	2015
关键词	HPLC-QTOF MS A549 Pteridinic profiling Pteridines Pteridine extraction EARLY CANCER-DETECTION HUMAN URINE TETRAHYDROBIOPTERIN BIOSYNTHESIS FLUOROMETRIC DETECTION BREAST-CANCER CELLS HPLC CREATININE BIOMARKERS NEOPTERIN
DOI	10.1016/j.aca.2014.10.044
英文摘要	Pteridines are a diverse family of endogenous metabolites that may serve as useful diagnostic biomarkers for disease. While many preparative and analytical techniques have been described for analysis of selected pteridines in biological fluids, broad intracellular pteridine detection remains a significant analytical challenge. In this study, a novel, specific and sensitive extraction and high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (HPLC-QTOF MS) method was developed to simultaneously quantify seven intracellular pteridines and monitor 18 additional, naturallyoccurring intracellular pteridines. The newly developed method was validated through evaluation of spiked recoveries (84.5-109.4%), reprodu cibility (2.1-5.4% RSD), method detection limits (0.1-3.0 mu g L-1) and limits of quantitation (0.1-1 mu g L-1), and finally application to non-small cell lung cancer A549 cells. Twenty-three pteridine derivatives were successfully detected from cell lysates with an average RSD of 12% among culture replicates. Quantified intracellular pteridine levels ranged from 1 to 1000 nM in good agreement with previous studies. Finally, this technique may be applied to cellular studies to generate new biological hypotheses concerning pteridine physiological and pathological functions as well as to discovery new pteridine-based biomarkers. (C) 2014 Elsevier B.V. All rights reserved.; Chemistry, Analytical; SCI(E); 0; ARTICLE; yu.bai@pku.edu.cn; yinfa@mst.edu; 442-450; 853
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/206726]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Burton, Casey,Weng, Rui,Yang, Li,et al. High-throughput intracellular pteridinic profiling by liquid chromatography-quadrupole time-of-flight mass spectrometry[J]. analytica chimica acta,2015.
APA	Burton, Casey,Weng, Rui,Yang, Li,Bai, Yu,Liu, Huwei,&Ma, Yinfa.(2015).High-throughput intracellular pteridinic profiling by liquid chromatography-quadrupole time-of-flight mass spectrometry.analytica chimica acta.
MLA	Burton, Casey,et al."High-throughput intracellular pteridinic profiling by liquid chromatography-quadrupole time-of-flight mass spectrometry".analytica chimica acta (2015).