Cyclization of several linear penta- and heptapeptides with different   metal ions studied by CD spectroscopy

doi:10.1111/j.1399-3011.2004.00199.x

CORC > 北京大学 > 化学与分子工程学院

	Cyclization of several linear penta- and heptapeptides with different metal ions studied by CD spectroscopy
	Liu, M ; Tang, YC ; Fan, KQ ; Jiang, X ; Lai, LH ; Ye, YH
刊名	journal of peptide research
	2005
关键词	CD spectra cyclic peptide cyclization DEPBT linear peptide metal ions STELLARIA-YUNNANENSIS CYCLIC HEXAPEPTIDE ENHANCED HELICITY COUPLING REAGENT PEPTIDE ESTERS GAS-PHASE CYCLOPEPTIDES CRYSTAL LIBRARY BINDING
DOI	10.1111/j.1399-3011.2004.00199.x
英文摘要	A cyclic pentapeptide c(Tyr-Leu-Ala-Gly-Pro) (I), which was isolated and identified from Pseudostellaria heterophylla medicinal herbs, and two cyclic heptapeptides, c(Gly-Tyr-Gly-Gly-Pro-Phe-Pro) (II) and c(Gly-Ile-Pro-Tyr-Ile-Ala-Ala) (III), which were isolated and identified from Stellaria yunnanensis Franch (M), were synthesized by using 3-(diethoxyphosphoryloxy)-1,2,3-benzotriazin-4(3 H)-one (DEPBT) as a coupling reagent in solution, and mediated by different metal ions, from their linear peptide precursors H-Tyr-Leu-Ala-Gly-Pro-OH (I-1) and H-Ala-Gly-Pro-Tyr-Leu-OH (I-2), H-Gly-Tyr-Gly-Gly-Pro-Phe-Pro-OH (II-1) and H-Gly-Ile-Pro-Tyr-Ile-Ala-Ala-OH (III-1), respectively. The results show that alkali metal ions can improve the cyclization yields and/or the cyclization rates of linear peptide precursors, such as Na+ ion is favorable for the cyclization of linear pentapeptides and Cs+ ion is favorable for the cyclization of linear heptapeptides, while some bivalent and trivalent metal ions, such as Mg2+, Ca2+, Zn2+, Fe2+, Ni2+ and Cr3+ reduced/inhibited both the cyclization yields and the cyclization rates of the linear peptide precursors. The circular dichroism spectra of I-1, II-1 and III-1 with different metal ions were studied to elucidate the changes in their secondary structures. It is shown that Cs+ can induce and stabilize the type I beta-turn conformation in the linear heptapeptide II-1 and the type II beta-turn conformation in the linear heptapeptide III-1.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000226707100008&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemical Research Methods; Biochemistry & Molecular Biology; SCI(E); PubMed; 8; ARTICLE; 1; 55-64; 65
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/199490]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Liu, M,Tang, YC,Fan, KQ,et al. Cyclization of several linear penta- and heptapeptides with different metal ions studied by CD spectroscopy[J]. journal of peptide research,2005.
APA	Liu, M,Tang, YC,Fan, KQ,Jiang, X,Lai, LH,&Ye, YH.(2005).Cyclization of several linear penta- and heptapeptides with different metal ions studied by CD spectroscopy.journal of peptide research.
MLA	Liu, M,et al."Cyclization of several linear penta- and heptapeptides with different metal ions studied by CD spectroscopy".journal of peptide research (2005).