Solution structure of the bacterial chemotaxis adaptor protein CheW from   Escherichia coli

doi:10.1016/j.bbrc.2007.06.146

CORC > 北京大学 > 化学与分子工程学院

	Solution structure of the bacterial chemotaxis adaptor protein CheW from Escherichia coli
	Li, You ; Hu, Yunfei ; Fu, Wenyu ; Xia, Bin ; Jin, Changwen
刊名	生物化学与生物物理学研究通讯
	2007
关键词	chemotaxis CheW solution structure NMR interaction NMR DYNAMICS SYSTEM KINASE
DOI	10.1016/j.bbrc.2007.06.146
英文摘要	The bacterial chemotaxis adaptor protein CheW physically links the chemoreceptors (MCPs) and the histidine kinase CheA. Extensive investigations using bacterium Escherichia coli have established the central role of CheW in the MCP-modulated activation of CheA. Here we report the solution structure of CheW from E. coli determined by NMR spectroscopy. The results show that E. coli CheW shares an overall fold with previously reported structure of CheW from Thermotoga maritima, whereas local conformational deviations are observed. In particular, the C-terminal alpha-helix is considerably longer in E. coli CheW and appears to shrink the active binding pocket with CheA. Our study provides the structural basis for further investigations in E. coli chemotaxis. (c) 2007 Elsevier Inc. All rights reserved.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000248502700026&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Biochemistry & Molecular Biology; Biophysics; SCI(E); PubMed; 11; ARTICLE; 4; 863-867; 360
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/198249]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Li, You,Hu, Yunfei,Fu, Wenyu,et al. Solution structure of the bacterial chemotaxis adaptor protein CheW from Escherichia coli[J]. 生物化学与生物物理学研究通讯,2007.
APA	Li, You,Hu, Yunfei,Fu, Wenyu,Xia, Bin,&Jin, Changwen.(2007).Solution structure of the bacterial chemotaxis adaptor protein CheW from Escherichia coli.生物化学与生物物理学研究通讯.
MLA	Li, You,et al."Solution structure of the bacterial chemotaxis adaptor protein CheW from Escherichia coli".生物化学与生物物理学研究通讯 (2007).