Estimation of binding constants of receptors and ligands by affinity   capillary electrophoresis

doi:10.1007/s00216-007-1167-5

CORC > 北京大学 > 化学与分子工程学院

	Estimation of binding constants of receptors and ligands by affinity capillary electrophoresis
	Zhang, Li-Wei ; Ding, Li ; Zhang, Xin-Xiang
刊名	分析化学和生物分析化学
	2007
关键词	affinity capillary electrophoresis binding constant retention factor migration time HUMAN SERUM-ALBUMIN ALANYL-D-ALANINE EQUILIBRIUM MIXTURES ELECTROKINETIC CHROMATOGRAPHY ANTIBIOTIC VANCOMYCIN PROTEIN-BINDING RISTOCETIN-A COMPLEX PEPTIDES BIOSYNTHESIS
DOI	10.1007/s00216-007-1167-5
英文摘要	An estimation method for determination of binding constants of receptors to ligands by affinity capillary electrophoresis was evaluated. On the basis of the theories of pseudostationary phase or so-called dynamic stationary phase, the retention factor (k) was used to represent the interaction between the receptor and ligand. k could be easily deduced from the migration times of the ligand and the receptor. Then, with the linear relationship of k versus the concentration of ligand in the running buffer, the binding constant K (b) was calculated from the slope and intercept. In order to test its feasibility, the calculation method was demonstrated using three model systems: the interactions between vancomycin and N-acetyl-Type=D-Ala-D-Ala, ristocetin and N-acetyl-D-Ala-D-Ala, and carbonic anhydrase B and an arylsulfonamide. Estimated binding constants were compared with those determined by other techniques. The results showed that this estimation method was reliable. This calculation method offers a simple and easy approach to estimating binding constants of ligands to receptors.; Biochemical Research Methods; Chemistry, Analytical; SCI(E); PubMed; 7; ARTICLE; 8; 2833-2841; 387
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/198188]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Zhang, Li-Wei,Ding, Li,Zhang, Xin-Xiang. Estimation of binding constants of receptors and ligands by affinity capillary electrophoresis[J]. 分析化学和生物分析化学,2007.
APA	Zhang, Li-Wei,Ding, Li,&Zhang, Xin-Xiang.(2007).Estimation of binding constants of receptors and ligands by affinity capillary electrophoresis.分析化学和生物分析化学.
MLA	Zhang, Li-Wei,et al."Estimation of binding constants of receptors and ligands by affinity capillary electrophoresis".分析化学和生物分析化学 (2007).