Investigate the role of PTEN in chemotaxis of human breast cancer cells

doi:10.1016/j.cellsig.2007.06.007

CORC > 北京大学 > 化学与分子工程学院

	Investigate the role of PTEN in chemotaxis of human breast cancer cells
	Wan, Wuzhou ; Zou, Haixia ; Sun, Ronghua ; Liu, Ying ; Wang, Jingna ; Ma, Dalong ; Zhang, Ning
刊名	cellular signalling
	2007
关键词	PTEN EGFR breast cancer chemotaxis adhesion metastasis KINASE-C-ZETA PROSTATE-CANCER METASTASIS MECHANISMS EXPRESSION POLARITY BRAIN MOUSE LEADS
DOI	10.1016/j.cellsig.2007.06.007
英文摘要	Chemotaxis plays an important role in metastasis of cancer cells. In the current study, we investigated the role of PTEN, a tumor suppressor, in chemotaxis of human breast cancer cells. Over-expression of PTEN inhibited EGF-induced chemotaxis, probably due to an overall reduction of PIP3 levels. Disruption of PTEN by siRNA caused a marked decrease in chemokinesis, cell adhesion, and membrane spreading, resulting in a severe defect in chemotaxis. In PTEN disrupted cells, PDK1, AKT, and PKC zeta exhibited elevated basal activities, which prevented EGF-induced further activation of these molecules. In the absence of EGF, active PDK1 was detected on multiple directions of the plasma membranes of PTEN disrupted cells, which competed against EGF-induced gradient sensing. To confirm the biological relevance of in vitro studies, both PTEN disrupted cells and its parental human breast cancer cells were injected into tail veins of SCID mice. Mice injected with PTEN disrupted cancer cells showed a marked decrease in lung metastasis. Taken together, our data show that PTEN plays a non-redundant role in EGF-induced chemotaxis of human breast cancer cells, and an optimal level of PTEN is required in these responses. (C) 2007 Published by Elsevier Inc.; http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000250026300003&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=8e1609b174ce4e31116a60747a720701 ; Cell Biology; SCI(E); PubMed; 14; REVIEW; 11; 2227-2236; 19
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/198119]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Wan, Wuzhou,Zou, Haixia,Sun, Ronghua,et al. Investigate the role of PTEN in chemotaxis of human breast cancer cells[J]. cellular signalling,2007.
APA	Wan, Wuzhou.,Zou, Haixia.,Sun, Ronghua.,Liu, Ying.,Wang, Jingna.,...&Zhang, Ning.(2007).Investigate the role of PTEN in chemotaxis of human breast cancer cells.cellular signalling.
MLA	Wan, Wuzhou,et al."Investigate the role of PTEN in chemotaxis of human breast cancer cells".cellular signalling (2007).