Study of the interactions between fluoroquinolones and human serum   albumin by affinity capillary electrophoresis and fluorescence method

doi:10.1016/j.aca.2007.09.021

CORC > 北京大学 > 化学与分子工程学院

	Study of the interactions between fluoroquinolones and human serum albumin by affinity capillary electrophoresis and fluorescence method
	Zhang, Li-Wei ; Wang, Kun ; Zhang, Xin-Xiang
刊名	analytica chimica acta
	2007
关键词	affinity capillary electrophoresis separation fluorescence method binding constant fluoroquinolones human serum albumin SOLID-PHASE EXTRACTION QUINOLONE ANTIBACTERIALS ZONE-ELECTROPHORESIS BINDING CONSTANTS PROTONATION EQUILIBRIA CHICKEN MUSCLE BOVINE ANTIBIOTICS SEPARATION ACID
DOI	10.1016/j.aca.2007.09.021
英文摘要	The interactions between fluoroquinolones and human serum albumin (HSA) were investigated by affinity capillary electrophoresis (ACE) and fluorescence quenching technique. Based on the efficient separation of several fluoroquinolones using a simple phosphate buffer, the binding constants of fluoroquinolones with HSA were determined simultaneously during one set of electrophoresis by ACE method. The thermodynamic parameters were obtained from data at different temperatures, and the negative Delta H and Delta S values showed that both hydrogen bonds and van der Waals interaction played major roles in the binding of fluoroquinolones to HSA. The interactions were also studied by fluorescence quenching technique. The results of fluorescence titration revealed that fluoroquinolones had the strong ability to quenching the intrinsic fluorescence of HSA through the static quenching procedure. The binding site number n, apparent binding constant K-b and the Stern-Volmer quenching constant K-sv were determined. The thermodynamic parameters were also studied by fluorescence method, and the results were consonant with that of ACE. (C) 2007 Elsevier B.V. All rights reserved.; Chemistry, Analytical; SCI(E); PubMed; 45; ARTICLE; 1; 101-110; 603
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/198032]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Zhang, Li-Wei,Wang, Kun,Zhang, Xin-Xiang. Study of the interactions between fluoroquinolones and human serum albumin by affinity capillary electrophoresis and fluorescence method[J]. analytica chimica acta,2007.
APA	Zhang, Li-Wei,Wang, Kun,&Zhang, Xin-Xiang.(2007).Study of the interactions between fluoroquinolones and human serum albumin by affinity capillary electrophoresis and fluorescence method.analytica chimica acta.
MLA	Zhang, Li-Wei,et al."Study of the interactions between fluoroquinolones and human serum albumin by affinity capillary electrophoresis and fluorescence method".analytica chimica acta (2007).