Direct immunoassay of estriol in pregnancy serum by capillary   electrophoresis with laser-induced fluorescence detector

doi:10.1016/S0039-9140(03)00179-6

CORC > 北京大学 > 化学与分子工程学院

	Direct immunoassay of estriol in pregnancy serum by capillary electrophoresis with laser-induced fluorescence detector
	Su, P ; Zhang, XX ; Wang, YC ; Chang, WB
刊名	talanta
	2003
关键词	estriol hydrogel laser-induced fluorescence capillary electrophoretic immunoassay PERFORMANCE LIQUID-CHROMATOGRAPHY THERMALLY REVERSIBLE HYDROGEL IMMUNOLOGICAL ANALYSIS ESTRADIOL POLARIZATION ANTIBODY AFFINITY
DOI	10.1016/S0039-9140(03)00179-6
英文摘要	A simple and sensitive capillary electrophoretic immunoassay (CETA) was described for the determination of estriol (E-3) in pregnant women's serum. The method was based on the competitive reaction of fluorescein-labeled E-3 antigen and E-3 with limited amounts of monoclonal antibody. The addition of the thermally reversible hydrogel, poly-N-iso propylacrylamide (pNIPA) in the buffer serving as a replaceable packing material, improved the reproducibility of the method. With laser-induced fluorescence detector (LIF), this method can be applied to determine E-3 at concentrations lower to 31.6 pg ml(-1). Recoveries from human steroid-free serum matrix were greater than 94% with relative standard deviation (R.S.D.) values less than 3.5%. Serum E-3 levels of ten normal pregnant women were measured at the range of 10.2-15.6 ng ml(-1). (C) 2003 Elsevier Science B.V. All rights reserved.; Chemistry, Analytical; SCI(E); EI; PubMed; 16; ARTICLE; 5; 969-975; 60
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/197729]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Su, P,Zhang, XX,Wang, YC,et al. Direct immunoassay of estriol in pregnancy serum by capillary electrophoresis with laser-induced fluorescence detector[J]. talanta,2003.
APA	Su, P,Zhang, XX,Wang, YC,&Chang, WB.(2003).Direct immunoassay of estriol in pregnancy serum by capillary electrophoresis with laser-induced fluorescence detector.talanta.
MLA	Su, P,et al."Direct immunoassay of estriol in pregnancy serum by capillary electrophoresis with laser-induced fluorescence detector".talanta (2003).