DegP primarily functions as a protease for the biogenesis of β-barrel outer membrane proteins in the Gram-negative bacterium Escherichia coli

doi:10.1111/febs.12701

CORC > 北京大学 > 化学与分子工程学院

	DegP primarily functions as a protease for the biogenesis of β-barrel outer membrane proteins in the Gram-negative bacterium Escherichia coli
	Ge Xi ; Wang Rui ; Ma Jing ; Liu Yang ; Ezemaduka Anastasia N ; Chen Peng R ; Fu Xinmiao ; Chang Zengyi
刊名	the febs journal
	2014
关键词	DegP,membrane protein biogenesis,protease,β-barrel outer membrane protein
DOI	10.1111/febs.12701
英文摘要	DegP (also designated as HtrA) and its homologs are found in prokaryotic cells and such eukaryotic organelles as mitochondria and chloroplasts. DegP has been found to be essential for the growth of Gram-negative bacteria under heat shock conditions and arguably considered to possess both protease and chaperone activities. The function of DegP has not been clearly defined. Using genetically incorporated non-natural amino acids as photo-crosslinkers, here we identified the β-barrel outer membrane proteins (OMPs) as the major natural substrates of DegP in Escherichia coli cells. We also demonstrated that DegP primarily functions as a protease, at both low and high temperatures, to eliminate unfolded OMPs, with hardly any appreciable chaperone activity in cells. We also found that the toxic and cell membrane-damaging misfolded OMPs would accumulate in DegP-lacking cells cultured under heat shock conditions. Together, our study defines the primary function of DegP in OMP biogenesis and offers a mechanistic insight into the essentiality of DegP for cell growth under heat shock conditions.? 2013 FEBS.; SCI(E); PubMed; 0; ARTICLE; fuxinmiao@pku.edu.cn; changzy@pku.edu.cn; 4; 1226-40; 281
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/190039]
专题	化学与分子工程学院生命科学学院
推荐引用方式 GB/T 7714	Ge Xi,Wang Rui,Ma Jing,et al. DegP primarily functions as a protease for the biogenesis of β-barrel outer membrane proteins in the Gram-negative bacterium Escherichia coli[J]. the febs journal,2014.
APA	Ge Xi.,Wang Rui.,Ma Jing.,Liu Yang.,Ezemaduka Anastasia N.,...&Chang Zengyi.(2014).DegP primarily functions as a protease for the biogenesis of β-barrel outer membrane proteins in the Gram-negative bacterium Escherichia coli.the febs journal.
MLA	Ge Xi,et al."DegP primarily functions as a protease for the biogenesis of β-barrel outer membrane proteins in the Gram-negative bacterium Escherichia coli".the febs journal (2014).