Highly sensitive detection of five typical fluoroquinolones in low-fat   milk by field-enhanced sample injection-based CE in bubble cell   capillary

doi:10.1002/elps.201400294

CORC > 北京大学 > 化学与分子工程学院

	Highly sensitive detection of five typical fluoroquinolones in low-fat milk by field-enhanced sample injection-based CE in bubble cell capillary
	Deng, Yan ; Gasilova, Natalia ; Qiao, Liang ; Zhou, Ying-Lin ; Zhang, Xin-Xiang ; Girault, Hubert H.
刊名	electrophoresis
	2014
关键词	Bubble cell capillary CE-electrostatic spray ionization MS Field-enhanced sample injection Fluoroquinolones Milk SOLID-PHASE EXTRACTION ELECTROPHORESIS-MASS-SPECTROMETRY ZONE-ELECTROPHORESIS LIQUID CHROMATOGRAPHY AMPEROMETRIC DETECTION FLUORESCENCE DETECTION ANTIBIOTICS RESIDUES CIPROFLOXACIN MS
DOI	10.1002/elps.201400294
英文摘要	Fluoroquinolones are a group of synthetic antibiotics with a broad activity spectrum against mycoplasma, Gram-positive, and Gram-negative bacteria. Due to the extensive use of fluoroquinolones in farming and veterinary science, there is a constant need in the analytical methods able to efficiently monitor their residues in food products of animal origin, regulated by Commission Regulation (European Union) no. 37/2010. Herein, field-enhanced sample injection for sample stacking prior the CZE separation was developed inside a bubble cell capillary for highly sensitive detection of five typical fluoroquinolones in bovine milk. Ethylenediamine was proposed as the main component of BGE for the antibiotics separation. The effect of BGE composition, injection parameters, and water plug length on the field-enhanced sample injection-based CE with UV detection was investigated. Under the optimized conditions, described field-enhanced sample injection-based CE-UV analysis of fluoroquinolones provides LODs varying from 0.4 to 1.3 ng/mL. These LOD values are much lower (from 460 to 1500 times) than those obtained by a conventional CE in a standard capillary without bubble cell. The developed method was finally applied for the analysis of fluoroquinolones in low-fat milk from a Swiss supermarket. Sample recovery values from 93.6 to 106.0% for different fluoroquinolones, and LODs from 0.7 to 2.5 mu g/kg, were achieved. Moreover, the proposed ethylenediamine-based BGE as volatile and compatible with MS system, enabled the coupling of the field-enhanced sample injection-based CE with a recently introduced electrostatic spray ionization MS via an iontophoretic fraction collection interface for qualitative fluoroquinolones identification.; Biochemical Research Methods; Chemistry, Analytical; SCI(E); PubMed; 1; ARTICLE; zxx@pku.edu.cn; hubert.girault@epfl.ch; 23; 3355-3362; 35
语种	英语
内容类型	期刊论文
源URL	[http://ir.pku.edu.cn/handle/20.500.11897/189248]
专题	化学与分子工程学院
推荐引用方式 GB/T 7714	Deng, Yan,Gasilova, Natalia,Qiao, Liang,et al. Highly sensitive detection of five typical fluoroquinolones in low-fat milk by field-enhanced sample injection-based CE in bubble cell capillary[J]. electrophoresis,2014.
APA	Deng, Yan,Gasilova, Natalia,Qiao, Liang,Zhou, Ying-Lin,Zhang, Xin-Xiang,&Girault, Hubert H..(2014).Highly sensitive detection of five typical fluoroquinolones in low-fat milk by field-enhanced sample injection-based CE in bubble cell capillary.electrophoresis.
MLA	Deng, Yan,et al."Highly sensitive detection of five typical fluoroquinolones in low-fat milk by field-enhanced sample injection-based CE in bubble cell capillary".electrophoresis (2014).